A1 Refereed original research article in a scientific journal
Metabolic Fate of C-13-Labeled Polydextrose and Impact on the Gut Microbiome: A Triple-Phase Study in a Colon Simulator
Authors: Lamichhane S, Yde CC, Jensen HM, Morovic W, Hibberd AA, Ouwehand AC, Saarinen MT, Forssten SD, Wiebe L, Marcussen J, Bertelsen K, Meier S, Young JF, Bertram HC
Publisher: AMER CHEMICAL SOC
Publication year: 2018
Journal: Journal of Proteome Research
Journal name in source: JOURNAL OF PROTEOME RESEARCH
Journal acronym: J PROTEOME RES
Volume: 17
Issue: 3
First page : 1041
Last page: 1053
Number of pages: 13
ISSN: 1535-3893
eISSN: 1535-3907
DOI: https://doi.org/10.1021/acs.jproteome.7b00683
Abstract
The present study introduces a novel triple-phase (liquids, solids, and gases) approach, which employed uniformly labeled [U-C-13] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing C-13 NMR spectroscopy, [U-C-13] PDX metabolism was observed from colonic digest samples. The major C-13-labeled metabolites generated were acetate, butyrate, propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), C-13-labeled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 h). Correlation analysis between C-13 NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level was statistically correlated with SCFA formation. Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
The present study introduces a novel triple-phase (liquids, solids, and gases) approach, which employed uniformly labeled [U-C-13] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing C-13 NMR spectroscopy, [U-C-13] PDX metabolism was observed from colonic digest samples. The major C-13-labeled metabolites generated were acetate, butyrate, propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), C-13-labeled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 h). Correlation analysis between C-13 NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level was statistically correlated with SCFA formation. Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
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