A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Both normal and leukemic B lymphocytes express multiple isoforms of the human Aiolos gene.
Tekijät: Liippo, Nera, Veistinen, Lähdesmäki, Postila, Kimby, Riikonen, Hammarström, Pelkonen, Lassila
Julkaisuvuosi: 2001
Journal: European Journal of Immunology
Tietokannassa oleva lehden nimi: European journal of immunology
Lehden akronyymi: Eur J Immunol
Vuosikerta: 31
Numero: 12
Aloitussivu: 3469
Lopetussivu: 74
Sivujen määrä: -3394
ISSN: 0014-2980
DOI: https://doi.org/10.1002/1521-4141(200112)31:12<3469::AID-IMMU3469>3.0.CO;2-G
Tiivistelmä
Aiolos is a chromatin remodeling transcription regulator that plays an antiproliferative role in B lymphocyte function. In contrast to the related Ikaros factors, mammalian Aiolos has not been reported to generate splice variants. In addition, although human leukemic lymphoblasts express non-DNA-binding Ikaros isoforms with potential dominant negative effect on other interacting factors,the role of Aiolos in human lymphoid disorders has remained obscure. To address the question, why Aiolos should delineate from Ikaros in such a marked way, we have here analyzed whether also human Aiolos could generate alternate isoforms. According to the results obtained, both normal and neoplastic B lineage cells were found to express at least five novel Aiolos variants. Also structurally dominant negative variants with less than three DNA-binding domains were identified. In conclusion, given the multiplicity of also human Aiolos isoforms and thereby the evidently more intricate contribution of Aiolos to the chromatin remodeling machinery, it is suggested, that not only Ikaros, but also Aiolos could participate in a more versatile manner in the regulation of B lymphocyte function.
Aiolos is a chromatin remodeling transcription regulator that plays an antiproliferative role in B lymphocyte function. In contrast to the related Ikaros factors, mammalian Aiolos has not been reported to generate splice variants. In addition, although human leukemic lymphoblasts express non-DNA-binding Ikaros isoforms with potential dominant negative effect on other interacting factors,the role of Aiolos in human lymphoid disorders has remained obscure. To address the question, why Aiolos should delineate from Ikaros in such a marked way, we have here analyzed whether also human Aiolos could generate alternate isoforms. According to the results obtained, both normal and neoplastic B lineage cells were found to express at least five novel Aiolos variants. Also structurally dominant negative variants with less than three DNA-binding domains were identified. In conclusion, given the multiplicity of also human Aiolos isoforms and thereby the evidently more intricate contribution of Aiolos to the chromatin remodeling machinery, it is suggested, that not only Ikaros, but also Aiolos could participate in a more versatile manner in the regulation of B lymphocyte function.
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