A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections




TekijätKrzyzanowska A, Lippolis G, Helczynski L, Anand A, Peltola M, Pettersson K, Lilja H, Bjartell A

KustantajaSAGE PUBLICATIONS LTD

Julkaisuvuosi2016

JournalJournal of Histochemistry and Cytochemistry

Tietokannassa oleva lehden nimiJOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Lehden akronyymiJ HISTOCHEM CYTOCHEM

Vuosikerta64

Numero5

Aloitussivu311

Lopetussivu322

Sivujen määrä12

ISSN0022-1554

eISSN1551-5044

DOIhttps://doi.org/10.1369/0022155416640466


Tiivistelmä

Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA intensity was significantly lower in cancer areas, particularly in AMACR- glands. The AR/PSA ratio varied significantly in the AMACR+ tumor cells as compared with benign glands. There was a trend of more rapid disease progression in patients with higher AR/PSA ratios in the AMACR- areas. This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions.




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