Pim-1 kinase and p100 cooperate to enhance c-myb activity



: Leverson JD, Koskinen PJ, Orrico FC, Rainio EM, Jalkanen KJ, Dash AB, Eisenman RN, Ness SA

Publisher: CELL PRESS

: 1998

: Molecular Cell

: MOLECULAR CELL

: MOL CELL

: 2

: 4

: 417

: 425

: 9

: 1097-2765

DOI: https://doi.org/10.1016/S1097-2765(00)80141-0


The pim-1 oncogene is regulated by hematopoietic cytokine receptors, encodes a serine/threonine protein kinase, and cooperates with c-myc in lymphoid cell transformation. Using a yeast two-hybrid screen, we found that pim-1 protein binds to p100, a transcriptional coactivator that interacts with the c-Myb transcription factor. Pim-1 phosphorylated p100 in vitro, formed a stable complex with p100 in animal cells, and functioned downstream of Ras to stimulate c-Myb transcriptional activity in a p100-dependent manner. Thus, pim-1 and p100 appear to be components of a novel signal transduction pathway affecting c-Myb activity, linking all three to the cytokine-regulated control of hematopoietic cell growth, differentiation, and apoptosis.

Last updated on 2024-26-11 at 21:38