A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A SUB-SET OF IMMEDIATE-EARLY MESSENGER-RNAS INDUCED BY TUMOR-NECROSIS-FACTOR-ALPHA DURING CELLULAR CYTOTOXIC AND NONCYTOTOXIC RESPONSES
Tekijät: KLEFSTRÖM J, KOSKINEN PJ, SAKSELA E, JÄÄTTELÄ M, BRAVO R, ALITALO K
Kustantaja: WILEY-LISS
Julkaisuvuosi: 1993
Journal: International Journal of Cancer
Tietokannassa oleva lehden nimi: INTERNATIONAL JOURNAL OF CANCER
Lehden akronyymi: INT J CANCER
Vuosikerta: 55
Numero: 4
Aloitussivu: 655
Lopetussivu: 659
Sivujen määrä: 5
ISSN: 0020-7136
DOI: https://doi.org/10.1002/ijc.2910550424
Tiivistelmä
TNF-alpha is a multifunctional cytokine which is cytotoxic for some cell lines. In order to characterize che early genomic response to TNF-alpha, we have analyzed the induction of a sub-set of serum-inducible immediate early genes in WEHI-S and L929 fibrosarcoma cell lines, which are sensitive to TNF-alpha, and in the 3T3-LI pre-adipocytic cell line, which is resistant to TNF-alpha cytotoxicity. Among 77 immediate early mRNAs screened by dot blot and/or Northern blot analyses, the expression of 23 mRNAs was found to be induced by TNF-alpha. Ten of these mRNAs encode proteins known to function as pro-inflammatory cytokines or transcription factors, while 13 others have as yet uncharacterized activities. The magnitude of c-fos induction by TNF-alpha inversely correlated with cell-type-specific cytotoxicity. Rapid and transient mRNA responses were observed in the TNF-alpha-resistant cells, whereas a slower and more persistent response was characteristic for TNF-alpha-sensitive cells. The prolonged induction of immediate early mRNAs may contribute to TNF-alpha-induced cellular cytotoxic responses. (C) 1993 Wiley-Liss, Inc.
TNF-alpha is a multifunctional cytokine which is cytotoxic for some cell lines. In order to characterize che early genomic response to TNF-alpha, we have analyzed the induction of a sub-set of serum-inducible immediate early genes in WEHI-S and L929 fibrosarcoma cell lines, which are sensitive to TNF-alpha, and in the 3T3-LI pre-adipocytic cell line, which is resistant to TNF-alpha cytotoxicity. Among 77 immediate early mRNAs screened by dot blot and/or Northern blot analyses, the expression of 23 mRNAs was found to be induced by TNF-alpha. Ten of these mRNAs encode proteins known to function as pro-inflammatory cytokines or transcription factors, while 13 others have as yet uncharacterized activities. The magnitude of c-fos induction by TNF-alpha inversely correlated with cell-type-specific cytotoxicity. Rapid and transient mRNA responses were observed in the TNF-alpha-resistant cells, whereas a slower and more persistent response was characteristic for TNF-alpha-sensitive cells. The prolonged induction of immediate early mRNAs may contribute to TNF-alpha-induced cellular cytotoxic responses. (C) 1993 Wiley-Liss, Inc.
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