A1 Journal article – refereed

Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library – Phage display derived HDL antibodies




Subtitle: Phage display derived HDL antibodies

List of Authors: Priyanka Negi,Janita Lövgren,Päivi Malmi,Nina Sirkka,Jari Metso,Tuomas Huovinen,Eeva-Christine Brockmann,Kim Pettersson,Matti Jauhiainen,Urpo Lamminmäki

Publisher: Elsevier

Publication year: 2016

Journal: Clinical Biochemistry

Journal acronym: Clin. Biochem

Volume number: 49

Issue number: 6

Number of pages: 8

ISSN: 0009-9120

eISSN: 1873-2933

DOI: http://dx.doi.org/10.1016/j.clinbiochem.2015.11.020


Abstract

Objective:In epidemiological studies plasma high density lipoprotein cholesterol (HDL-C) levels are found to correlate inversely with atherosclerotic cardiovascular events. HDL consists of different subpopulations and they vary in their anti-atherogenic properties. The aim of this study is to isolate coronary artery disease (CAD) specific anti-HDL scFv-antibodies

Design and Methods:To obtain CAD specific HDL binders, we used phage displayed synthetic antibody libraries to enrich specific antibodies against HDL isolated from CAD patients. The antibodies were affinity purified. Their capability to recognize apolipoproteins A-I and A-II, various HDL forms differing in lipid/protein ratios and plasma HDL was studied using time-resolved fluorescence based immunoassay.

Results:

Using different selection strategies and immunoassay based screening we obtained altogether 1200 clones displaying HDL binding activity. By sequencing 337 we identified 264 unique antibodies against HDL. A set of 61 antibodies were selected for further analysis. We found a variety of antibodies with different binding profiles, including apoA-I binding antibodies either in lipid-dependent or lipid-independent manner and binders against apoA-II. Several antibodies were able to discriminate between HDL derived from CAD patients and healthy controls. A majority of the antibodies were immunoreactive with HDL in plasma.

Conclusion:The novel HDL recognizing antibodies isolated from synthetic antibody phage library have displayed interesting HDL-binding characteristics suggesting that, in addition to use as research tools, a part of them might be useful for the development of diagnostic methods for CAD risk assessment.



Last updated on 2021-24-06 at 11:07