A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Determination of the nucleotide conformation in the productive enzyme-substrate complexes of RNA-depolymerases
Tekijät: Moiseyev GP, Yakovlev GI, Lysov YP, Chernyi AA, Polyakov KM, Oivanen M, Lonnberg H, Beigelman LN, Efimtseva EV, Mikhailov SN
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 1997
Lehti:: FEBS Letters
Tietokannassa oleva lehden nimi: FEBS LETTERS
Lehden akronyymi: FEBS LETT
Vuosikerta: 404
Numero: 2-3
Aloitussivu: 169
Lopetussivu: 172
Sivujen määrä: 4
ISSN: 0014-5793
DOI: https://doi.org/10.1016/S0014-5793(97)00092-6
Tiivistelmä
The aim of this work is to determine the conformation of the nucleobase adjacent to the cleavable phosphodiester bond in the productive enzyme-substrate complex of RNA-depolymerizing enzymes, To this end the kinetic parameters of hydrolysis of UpA, 2'-C-Me- and 3'-C-Me-UpA were determined for RNase A, RNase Pb-2, nuclease S-1 and snake venom phosphodiesterase, In these derivatives the ranges of the allowed orientation of uridine residues are restricted due to the substitution of methyl groups for the ribose hydrogen atoms, The results described demonstrate that the proposed method is of general value for the estimation of the nucleotide glycoside angles in the productive enzyme-substrate complexes. (C) 1997 Federation of European Biochemical Societies.
The aim of this work is to determine the conformation of the nucleobase adjacent to the cleavable phosphodiester bond in the productive enzyme-substrate complex of RNA-depolymerizing enzymes, To this end the kinetic parameters of hydrolysis of UpA, 2'-C-Me- and 3'-C-Me-UpA were determined for RNase A, RNase Pb-2, nuclease S-1 and snake venom phosphodiesterase, In these derivatives the ranges of the allowed orientation of uridine residues are restricted due to the substitution of methyl groups for the ribose hydrogen atoms, The results described demonstrate that the proposed method is of general value for the estimation of the nucleotide glycoside angles in the productive enzyme-substrate complexes. (C) 1997 Federation of European Biochemical Societies.
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