A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
In vivo sampling for pharmacokinetic studies in small experimental animals: A combination of microdialysis, planar chromatography and digital autoradiography
Tekijät: Haaparanta M, Gronroos T, Marjamaki P, Eskola I, Bergman J, Paul R, Solin O
Kustantaja: ELSEVIER SCIENCE INC
Julkaisuvuosi: 2004
Tietokannassa oleva lehden nimi: MOLECULAR IMAGING AND BIOLOGY
Lehden akronyymi: MOL IMAGING BIOL
Vuosikerta: 6
Numero: 1
Aloitussivu: 27
Lopetussivu: 33
Sivujen määrä: 7
ISSN: 1536-1632
DOI: https://doi.org/10.1016/j.mibio.2003.12.005
Tiivistelmä
PURPOSE: We describe a method for measuring the pharmacokinetics of positron emission tomography (PET) tracers during rat studies using microdialysis (MD) in combination with planar chromatography and digital autoradiography with a phosphoimager plate (DAR).PROCEDURES: An MD probe was inserted into the jugular vein of the rat and perfused with physiological buffer solution (n greater than or equal to 3). An F-18-labeled radiopharmaceutical was injected intravenously, and dialysate fractions were collected, measured for radioactivity, and applied on a chromatography plate. This plate was exposed with an imaging plate for radioactivity imaging.RESULTS: Time activity curves for unbound, unchanged tracer and metabolites were determined. The amount of unchanged tracer in the last MD fraction was compared with the amount in the plasma sample collected after sacrifice of the animal.CONCLUSION: MD continuously samples and monitors the amount of unbound tracer in the blood from one single experimental animal. MD combined with planar chromatography and DAR is a highly sensitive and linear method for simultaneous analysis of PET tracers and their metabolites in blood. (C) 2004 Elsevier Inc. All rights reserved.
PURPOSE: We describe a method for measuring the pharmacokinetics of positron emission tomography (PET) tracers during rat studies using microdialysis (MD) in combination with planar chromatography and digital autoradiography with a phosphoimager plate (DAR).PROCEDURES: An MD probe was inserted into the jugular vein of the rat and perfused with physiological buffer solution (n greater than or equal to 3). An F-18-labeled radiopharmaceutical was injected intravenously, and dialysate fractions were collected, measured for radioactivity, and applied on a chromatography plate. This plate was exposed with an imaging plate for radioactivity imaging.RESULTS: Time activity curves for unbound, unchanged tracer and metabolites were determined. The amount of unchanged tracer in the last MD fraction was compared with the amount in the plasma sample collected after sacrifice of the animal.CONCLUSION: MD continuously samples and monitors the amount of unbound tracer in the blood from one single experimental animal. MD combined with planar chromatography and DAR is a highly sensitive and linear method for simultaneous analysis of PET tracers and their metabolites in blood. (C) 2004 Elsevier Inc. All rights reserved.
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