A1 Refereed original research article in a scientific journal
The binding capacity of alpha 1 beta 1-, alpha 2 beta 1-and alpha 10 beta 1-integrins depends on non-collagenous surface macromolecules rather than the collagens in cartilage fibrils
Authors: Woltersdorf C, Bonk M, Leitinger B, Huhtala M, Kapyla J, Heino J, Girol CG, Niland S, Eble JA, Bruckner P, Dreier R, Hansen U
Publisher: ELSEVIER SCIENCE BV
Publication year: 2017
Journal: Matrix Biology
Journal name in source: MATRIX BIOLOGY
Journal acronym: MATRIX BIOL
Volume: 63
First page : 91
Last page: 105
Number of pages: 15
ISSN: 0945-053X
eISSN: 1569-1802
DOI: https://doi.org/10.1016/j.matbio.2017.02.001
Abstract
Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of beta 1-integrins with von Willebrand-factor A-like domains (I-domains) in their alpha-chains can bind to collagen molecules and, therefore, are considered as important cellular mechano-receptors. Here we show that chondrocytes strongly bind to cartilage collagens in the form of individual triple helical molecules but very weakly to fibrils formed by the same molecules. We also find that chondrocyte integrins alpha 1 beta 1-, alpha 2 beta 1- and alpha 10 beta 1-integrins and their I-domains have the same characteristics. Nevertheless we find integrin binding to mechanically generated cartilage fibril fragments, which also comprise peripheral non-collagenous material. We conclude that cell adhesion results from binding of integrin-containing adhesion suprastructures to the non-collagenous fibril periphery but not to the collagenous fibril cores. The biological importance of the well-investigated recognition of collagen molecules by integrins is unknown. Possible scenarios may include fibrillogenesis, fibril degradation and/or phagocytosis, recruitment of cells to remodeling sites, or molecular signaling across cytoplasmic membranes. In these circumstances, collagen molecules may lack a fibrillar organization. However, other processes requiring robust biomechanical functions, such as fibril organization in tissues, cell division, adhesion, or migration, do not involve direct integrin-collagen interactions. (C) 2017 Elsevier B.V. All rights reserved.
Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of beta 1-integrins with von Willebrand-factor A-like domains (I-domains) in their alpha-chains can bind to collagen molecules and, therefore, are considered as important cellular mechano-receptors. Here we show that chondrocytes strongly bind to cartilage collagens in the form of individual triple helical molecules but very weakly to fibrils formed by the same molecules. We also find that chondrocyte integrins alpha 1 beta 1-, alpha 2 beta 1- and alpha 10 beta 1-integrins and their I-domains have the same characteristics. Nevertheless we find integrin binding to mechanically generated cartilage fibril fragments, which also comprise peripheral non-collagenous material. We conclude that cell adhesion results from binding of integrin-containing adhesion suprastructures to the non-collagenous fibril periphery but not to the collagenous fibril cores. The biological importance of the well-investigated recognition of collagen molecules by integrins is unknown. Possible scenarios may include fibrillogenesis, fibril degradation and/or phagocytosis, recruitment of cells to remodeling sites, or molecular signaling across cytoplasmic membranes. In these circumstances, collagen molecules may lack a fibrillar organization. However, other processes requiring robust biomechanical functions, such as fibril organization in tissues, cell division, adhesion, or migration, do not involve direct integrin-collagen interactions. (C) 2017 Elsevier B.V. All rights reserved.
UTU Research Portal