A1 Refereed original research article in a scientific journal
Use of differential expression data for identification of novel immune relevant expressed sequence tag-linked microsatellite markers in Atlantic salmon (Salmo salar L.)
Authors: Tonteri A, Vasemagi A, Lumme J, Primmer CR
Publisher: BLACKWELL PUBLISHING
Publication year: 2008
Journal: Molecular Ecology Resources
Journal name in source: MOLECULAR ECOLOGY RESOURCES
Journal acronym: MOL ECOL RESOUR
Volume: 8
Issue: 6
First page : 1486
Last page: 1490
Number of pages: 5
ISSN: 1755-098X
DOI: https://doi.org/10.1111/j.1755-0998.2008.02317.x(external)
Abstract
Despite the large number of genes contributing to the immune response, wildlife immunogenetic studies have tended to focus mostly on the major histocompatibility complex-related genes. Here, we utilized previously published microarray and competitive RNA hybridization information to identify 3750 immune relevant Atlantic salmon (Salmo salar) expressed sequence tags. We then identified those expressed sequence tags containing microsatellites and subsequently designed 48 primer pairs and tested them for polymorphism in Atlantic salmon. Altogether, 16 polymorphic markers were characterized, with allele numbers ranging from two to 18, and these 16 loci were further tested in five other salmonid species.
Despite the large number of genes contributing to the immune response, wildlife immunogenetic studies have tended to focus mostly on the major histocompatibility complex-related genes. Here, we utilized previously published microarray and competitive RNA hybridization information to identify 3750 immune relevant Atlantic salmon (Salmo salar) expressed sequence tags. We then identified those expressed sequence tags containing microsatellites and subsequently designed 48 primer pairs and tested them for polymorphism in Atlantic salmon. Altogether, 16 polymorphic markers were characterized, with allele numbers ranging from two to 18, and these 16 loci were further tested in five other salmonid species.
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