A4 Refereed article in a conference publication
Photo-induced ultrasound microscopy for photo-acoustic imaging of non-absorbing specimens
Authors: Tcarenkova E, Koho SV, Hanninen PE
Editors: Costa MFM
Conference name: International Conference on Applications of Optics and Photonics
Publisher: SPIE-INT SOC OPTICAL ENGINEERING, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Publication year: 2017
Book title : Third International Conference on Applications of Optics and Photonics
Journal name in source: THIRD INTERNATIONAL CONFERENCE ON APPLICATIONS OF OPTICS AND PHOTONICS
Journal acronym: PROC SPIE
Article number: UNSP 104532O
Series title: Proceedings of SPIE
Volume: 10453
Number of pages: 6
ISBN: 978-1-5106-1384-3
ISSN: 0277-786X
DOI: https://doi.org/10.1117/12.2276315
Photo-Acoustic Microscopy (PAM) has raised high interest in in-vivo imaging due to its ability to preserve the near-diffraction limited spatial resolution of optical microscopes, whilst extending the penetration depth to the mm-range. Another advantage of PAM is that it is a label-free technique - any substance that absorbs PAM excitation laser light can be viewed. However, not all sample structures desired to be observed absorb sufficiently to provide contrast for imaging. This work describes a novel imaging method that makes it possible to visualize optically transparent samples that lack intrinsic photo-acoustic contrast, without the addition of contrast agents. A thin, strongly light absorbing layer next to sample is used to generate a strong ultrasonic signal. This signal, when recorded from opposite side, contains ultrasonic transmission information of the sample and thus the method can be used to obtain an ultrasound transmission image on any PAM.
