A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
TPA primes alpha 2 beta 1 integrins for cell adhesion
Tekijät: Tulla M, Helenius J, Jokinen J, Taubenberger A, Muller DJ, Heino J
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2008
Lehti:: FEBS Letters
Tietokannassa oleva lehden nimi: FEBS LETTERS
Lehden akronyymi: FEBS LETT
Vuosikerta: 582
Numero: 23-24
Aloitussivu: 3520
Lopetussivu: 3524
Sivujen määrä: 5
ISSN: 0014-5793
DOI: https://doi.org/10.1016/j.febslet.2008.09.022
Tiivistelmä
Integrin avidity is regulated by changes in the conformation of the heterodimer and cluster formation. We measured cell adhesion by integrin alpha 2 beta 1 (CHO-alpha 2) to collagen at short contact times (0.5-60 s) by single cell force spectroscopy (SCFS). The adhesion increased rapidly with contact time and was further strengthened by the addition of 12-O-tetradecanoylphorbol-13- acetate (TPA), a protein kinase C (PKC) and integrin activator. TPA also improved the strength of adhesive units. Furthermore, changes in membrane nanotube properties indicated better coupling of integrins to the cell cytoskeleton. We conclude that in addition to increasing integrin avidity TPA strengthens integrin-cytoskeletal linkage.
Integrin avidity is regulated by changes in the conformation of the heterodimer and cluster formation. We measured cell adhesion by integrin alpha 2 beta 1 (CHO-alpha 2) to collagen at short contact times (0.5-60 s) by single cell force spectroscopy (SCFS). The adhesion increased rapidly with contact time and was further strengthened by the addition of 12-O-tetradecanoylphorbol-13- acetate (TPA), a protein kinase C (PKC) and integrin activator. TPA also improved the strength of adhesive units. Furthermore, changes in membrane nanotube properties indicated better coupling of integrins to the cell cytoskeleton. We conclude that in addition to increasing integrin avidity TPA strengthens integrin-cytoskeletal linkage.
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