Refereed journal article or data article (A1)

Interaction of cholera toxin B subunit with T and B lymphocytes




List of AuthorsNavolotskaya EV, Sadovnikov VB, Zitichenko DV, Lipkin VM, Zav'yalov VP

PublisherELSEVIER SCIENCE BV

Publication year2017

JournalInternational Immunopharmacology

Journal name in sourceINTERNATIONAL IMMUNOPHARMACOLOGY

Journal acronymINT IMMUNOPHARMACOL

Volume number50

Start page279

End page282

Number of pages4

ISSN1567-5769

eISSN1878-1705

DOIhttp://dx.doi.org/10.1016/j.intimp.2017.07.011


Abstract
We have prepared I-125-labeled cholera toxin B subunit (I-125-labeled CT-B, a specific activity of 98 Ci/mmol) and found that its binding to T and B lymphocytes from the blood of healthy donors was high-affinity (K-d 2.8 and 3.0 nM, respectively). The binding of labeled protein was completely inhibited by unlabeled thymosin-alpha(1) (TM-alpha(1)), interferon-alpha(2) (IFN-alpha(2)), and the synthetic peptide LKEKK that corresponds to residues 16-20 in TM-alpha(1) and 131-135 in IFN-alpha(2), but was not inhibited by the synthetic peptide KKEKL with inverted amino acid sequence (K-1 > 10 mu M). Thus, TM-alpha(1), IFN-alpha(2), and the peptide: LKEKK bind with high affinity and specificity to CT-B receptor on donor blood T and B lymphocytes. It was found that CT-B and the peptide: LKEKK at concentrations of 10-1000 nM increased in a dose-dependent manner the soluble guanylate cyclase activity in T and B lymphocytes.


Last updated on 2021-24-06 at 09:29