A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Tagging of phosphopeptides by phosphate elimination and Michael addition: The effect of nearest neighbors on the reaction kinetics
Tekijät: Mattila K, Ora M, Lonnberg H
Kustantaja: BENTHAM SCIENCE PUBL LTD
Julkaisuvuosi: 2006
Lehti:: Letters in Organic Chemistry
Tietokannassa oleva lehden nimi: LETTERS IN ORGANIC CHEMISTRY
Lehden akronyymi: LETT ORG CHEM
Vuosikerta: 3
Numero: 7
Aloitussivu: 514
Lopetussivu: 518
Sivujen määrä: 5
ISSN: 1570-1786
DOI: https://doi.org/10.2174/157017806778341924
Tiivistelmä
Kinetics of the base-catalyzed elimination of the phosphate group from short phosphoserinyl peptides and subsequent addition of beta-mercaptoethanol to the dehydroalaninyl residue have been studied by following the reactions by RP HPLC in an 8:3:1 (vlvlv) mixture of water, DMSO and ethanol. The data obtained shows that neighboring side-chain-functionalized residues, including Asp, Tyr, Lys, His, Pro and Asn, influence the rate of elimination and addition steps by less than one order of magnitude. The amount of byproducts always remains below 10 %, lending support to the applicability of the method as a research tool for functional phosphoproteornics.
Kinetics of the base-catalyzed elimination of the phosphate group from short phosphoserinyl peptides and subsequent addition of beta-mercaptoethanol to the dehydroalaninyl residue have been studied by following the reactions by RP HPLC in an 8:3:1 (vlvlv) mixture of water, DMSO and ethanol. The data obtained shows that neighboring side-chain-functionalized residues, including Asp, Tyr, Lys, His, Pro and Asn, influence the rate of elimination and addition steps by less than one order of magnitude. The amount of byproducts always remains below 10 %, lending support to the applicability of the method as a research tool for functional phosphoproteornics.
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