A1 Refereed original research article in a scientific journal
Inflammatory Stroke Extracellular Vesicles Induce Macrophage Activation
Authors: Couch Y, Akbar N, Davis S, Fischer R, Dickens AM, Neuhaus AA, Burgess AI, Rothwell PM, Buchan AM
Publisher: LIPPINCOTT WILLIAMS & WILKINS
Publication year: 2017
Journal: Stroke
Journal name in source: STROKE
Journal acronym: STROKE
Volume: 48
Issue: 8
First page : 2292
Last page: 2296
Number of pages: 8
ISSN: 0039-2499
eISSN: 0039-2499
DOI: https://doi.org/10.1161/STROKEAHA.117.017236
Abstract
Background and Purpose-Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs.Methods-EVs were quantified and analyzed in the sera of patients after an acute stroke (< 24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography massspectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression.Results-EV number, but not size, is significantly increased in stroke patients when compared to age-matched controls. Proteomic analysis reveals an overall increase in acute phase proteins, including C-reactive protein. EV fractions applied to monocyte-differentiated macrophage cultures induced inflammatory gene expression.Conclusions-Together these data show that EVs from stroke patients are proinflammatory in nature and are capable of inducing inflammation in immune cells.
Background and Purpose-Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs.Methods-EVs were quantified and analyzed in the sera of patients after an acute stroke (< 24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography massspectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression.Results-EV number, but not size, is significantly increased in stroke patients when compared to age-matched controls. Proteomic analysis reveals an overall increase in acute phase proteins, including C-reactive protein. EV fractions applied to monocyte-differentiated macrophage cultures induced inflammatory gene expression.Conclusions-Together these data show that EVs from stroke patients are proinflammatory in nature and are capable of inducing inflammation in immune cells.
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