A1 Refereed original research article in a scientific journal
The effect of secondary structure on cleavage of the phosphodiester bonds of RNA
Authors: Mikkola S, Kaukinen U, Lonnberg H
Publisher: HUMANA PRESS INC
Publication year: 2001
Journal: Cell Biochemistry and Biophysics
Journal name in source: CELL BIOCHEMISTRY AND BIOPHYSICS
Journal acronym: CELL BIOCHEM BIOPHYS
Volume: 34
Issue: 1
First page : 95
Last page: 119
Number of pages: 25
ISSN: 1085-9195
DOI: https://doi.org/10.1385/CBB:34:1:95
Abstract
This review discusses the effects the secondary structure of an RNA molecule has on the inherent reactivity of its phosphodiester bonds, and on the catalytic activity of metal ion-based cleaving agents. The basic principles of the intramolecular transesterification of RNA phosphodiester bonds, particularly cleavage, are first briefly described. Studies of the structural effects on the cleavage, in the absence and in the presence of metal ion catalysts, are then reviewed, and the sources of the reactivity differences observed in different structures are discussed.
This review discusses the effects the secondary structure of an RNA molecule has on the inherent reactivity of its phosphodiester bonds, and on the catalytic activity of metal ion-based cleaving agents. The basic principles of the intramolecular transesterification of RNA phosphodiester bonds, particularly cleavage, are first briefly described. Studies of the structural effects on the cleavage, in the absence and in the presence of metal ion catalysts, are then reviewed, and the sources of the reactivity differences observed in different structures are discussed.
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