Phosphodiester cleavage of diribonucleoside 3 ',3 '-monophosphates: Rate acceleration by the 2 '-hydroxyl and 2 '-amino function, implications to ribozyme action
: Ora M, Kiviniemi A, Lonnberg T, Virtanen S, Lonnberg H
: XIII Symposium on Chemistry of Nucleic Acid Components
: 2005
Collection Symposium Series
Chemistry of Nucleic Acid Components
: COLL SYMPOS SERIES
: 7
: 261
: 263
: 3
: 80-86241-25-4
The hydrolytic reactions of diribonucleoside 3',3'-monophosphates have been followed by RP HPLC over a wide pH-range at 363.2 K. The mechanisms of the reactions are discussed, particularly focusing on the possible stabilization of phosphorane intermediate and/or transition state via an intramolecular hydrogen bonding by the 2'-hydroxyl and 2'-amino group.
