A2 Vertaisarvioitu katsausartikkeli tieteellisessä lehdessä
High sensitivity luminescence nanoparticle assay for the detection of protein aggregation
Tekijät: Pihlasalo S, Kirjavainen J, Hänninen P, Härmä H
Kustantaja: American Chemical Society
Julkaisuvuosi: 2011
Journal: Analytical Chemistry
Tietokannassa oleva lehden nimi: Analytical Chemistry
Lehden akronyymi: ANAL CHEM
Numero sarjassa: 4
Vuosikerta: 83
Numero: 4
Aloitussivu: 1163
Lopetussivu: 1166
Sivujen määrä: 4
ISSN: 0003-2700
DOI: https://doi.org/10.1021/ac1026385
Tiivistelmä
Nanoparticle assay utilizing time-resolved luminescence resonance energy transfer (TR-LRET) was developed for the detection of protein aggregation. This mix-and-measure nanoparticle assay is based on the competitive adsorption of the sample and the acceptor-labeled protein to donor europium(III) polystyrene particles. The protein aggregation was detected with the developed TR-LRET nanoparticle assay, UV240 absorbance and dynamic light scattering (DLS). All methods well equally detected the aggregation and aggregates, whose size ranged from single protein to more than 1000 nm aggregates. The developed method allowed the aggregation detection of the entire size range at more than 10 000 times lower concentration, 30 μg/L, compared to UV240 and DLS. The simple-to-use and sensitive nanoparticle assay with existing microtiter plate luminometric instrumentation can find use as a routine tool for protein aggregation studies in biochemical laboratories and for quality assessment of protein products in industry. © 2011 American Chemical Society.
Nanoparticle assay utilizing time-resolved luminescence resonance energy transfer (TR-LRET) was developed for the detection of protein aggregation. This mix-and-measure nanoparticle assay is based on the competitive adsorption of the sample and the acceptor-labeled protein to donor europium(III) polystyrene particles. The protein aggregation was detected with the developed TR-LRET nanoparticle assay, UV240 absorbance and dynamic light scattering (DLS). All methods well equally detected the aggregation and aggregates, whose size ranged from single protein to more than 1000 nm aggregates. The developed method allowed the aggregation detection of the entire size range at more than 10 000 times lower concentration, 30 μg/L, compared to UV240 and DLS. The simple-to-use and sensitive nanoparticle assay with existing microtiter plate luminometric instrumentation can find use as a routine tool for protein aggregation studies in biochemical laboratories and for quality assessment of protein products in industry. © 2011 American Chemical Society.
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