A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Development of an optical surface plasmon resonance biosensor assay for (fluoro)quinolones in egg, fish, and poultry meat
Tekijät: Huet AC, Charlier C, Singh G, Godefroy SB, Leivo J, Vehniaeinen M, Nielen MWF, Weigel S, Delahaut P
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2008
Lehti:Analytica Chimica Acta
Tietokannassa oleva lehden nimiANALYTICA CHIMICA ACTA
Lehden akronyymi: ANAL CHIM ACTA
Vuosikerta: 623
Numero: 2
Aloitussivu: 195
Lopetussivu: 203
Sivujen määrä: 9
ISSN: 0003-2670
DOI: https://doi.org/10.1016/j.aca.2008.06.009
Tiivistelmä
The aim of this study was to develop an optical biosensor inhibition immunoassay, based on the surface plasmon resonance (SPR) principle, for use as a screening test for 13 (fluoro)quinolones, including flumequine, used as veterinary drugs in food-producing animals. For this, we immobilised various quinolone derivatives on the sensor chip and tested binding of a range of different antibodies (polyclonal and one engineered antibody) in the presence and absence of free (fluoro)quinolones. The main challenge was to detect flumequine in an assay giving good results for the other compounds. One antigen-antibody combination proved satisfactory: polyclonal antibodies raised against a dual immunogen and, on the sensor chip, a fluoroquinolone derivative. It was the first time that this concept of the bi-active antibody was described in the literature. The assay, optimised for detection in three matrices (poultry muscle, fish, and egg), was tested on incurred samples prepared by liquid extraction followed by two washing steps. This rapid, simple method proved adequate for detecting at least 13 (fluoro)quinolones at concentrations below established maximum residue levels (MRLs). The reference molecule norfloxacin could be detected in the range of 0.1-10 mu g kg(-1) in extracts of egg and poultry meat and in the range of 0.1-100 mu g kg(-1) in extracts of fish. The determined midpoints of these calibration curves were about 1, 1.5 and 3 mu g kg(-1) in poultry meat, egg and fish, respectively. (C) 2008 Elsevier B.V. All rights reserved.
The aim of this study was to develop an optical biosensor inhibition immunoassay, based on the surface plasmon resonance (SPR) principle, for use as a screening test for 13 (fluoro)quinolones, including flumequine, used as veterinary drugs in food-producing animals. For this, we immobilised various quinolone derivatives on the sensor chip and tested binding of a range of different antibodies (polyclonal and one engineered antibody) in the presence and absence of free (fluoro)quinolones. The main challenge was to detect flumequine in an assay giving good results for the other compounds. One antigen-antibody combination proved satisfactory: polyclonal antibodies raised against a dual immunogen and, on the sensor chip, a fluoroquinolone derivative. It was the first time that this concept of the bi-active antibody was described in the literature. The assay, optimised for detection in three matrices (poultry muscle, fish, and egg), was tested on incurred samples prepared by liquid extraction followed by two washing steps. This rapid, simple method proved adequate for detecting at least 13 (fluoro)quinolones at concentrations below established maximum residue levels (MRLs). The reference molecule norfloxacin could be detected in the range of 0.1-10 mu g kg(-1) in extracts of egg and poultry meat and in the range of 0.1-100 mu g kg(-1) in extracts of fish. The determined midpoints of these calibration curves were about 1, 1.5 and 3 mu g kg(-1) in poultry meat, egg and fish, respectively. (C) 2008 Elsevier B.V. All rights reserved.
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