A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Comparative stability of dimeric and monomeric pigments extracted from sea urchin Strongylocentrotus droebachiensis
Tekijät: Shikov AN, Ossipov VI, Karonen M, Pozharitskaya ON, Krishtopina AS, Makarov VG
Kustantaja: TAYLOR & FRANCIS LTD
Julkaisuvuosi: 2017
Journal: Natural Product Research
Tietokannassa oleva lehden nimi: NATURAL PRODUCT RESEARCH
Lehden akronyymi: NAT PROD RES
Vuosikerta: 31
Numero: 15
Aloitussivu: 1747
Lopetussivu: 1751
Sivujen määrä: 5
ISSN: 1478-6419
eISSN: 1478-6427
DOI: https://doi.org/10.1080/14786419.2017.1290617
Tiivistelmä
Ultraviolet-visible spectroscopy and UPLC-DAD-MS were used for analysis of stability of ethanol solutions of ethylidene-6,6-bis(2,3,7-trihydroxynaphthazarin) (ENZ), spinochrome dimer (SDM) and spinochrome D (SD) that were isolated from Strongylocentrotus droebachiensis. In the freshly prepared solution, the concentration of ENZ at pH 6.0 was at 6 fold less comparing to pH 1.6. The increase of pH up to 4.0 resulted to increase of SD concentration and to decrease of SDM concentration. After 48h storage, both dimers showed the highest stability at pH 1.6, while the elevation of the pH solution up to 6.0 activates degradation of SDM and ENZ at 1.3 and 3.6 fold correspondingly. The concentration of SD after 48h storage at the pH 1.6 was at two-fold less comparing to the initial concentration, and at the pH 6.0 - at 4 fold less. This study contributes to increasing the knowledge on the stability of the spinochrome pigments.[GRAPHICS].
Ultraviolet-visible spectroscopy and UPLC-DAD-MS were used for analysis of stability of ethanol solutions of ethylidene-6,6-bis(2,3,7-trihydroxynaphthazarin) (ENZ), spinochrome dimer (SDM) and spinochrome D (SD) that were isolated from Strongylocentrotus droebachiensis. In the freshly prepared solution, the concentration of ENZ at pH 6.0 was at 6 fold less comparing to pH 1.6. The increase of pH up to 4.0 resulted to increase of SD concentration and to decrease of SDM concentration. After 48h storage, both dimers showed the highest stability at pH 1.6, while the elevation of the pH solution up to 6.0 activates degradation of SDM and ENZ at 1.3 and 3.6 fold correspondingly. The concentration of SD after 48h storage at the pH 1.6 was at two-fold less comparing to the initial concentration, and at the pH 6.0 - at 4 fold less. This study contributes to increasing the knowledge on the stability of the spinochrome pigments.[GRAPHICS].
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