Novel non-nucleosidic building blocks for the preparation of multilabeled oligonucleotides



: Guzaev A, Salo H, Azhayev A, Lonnberg H

Publisher: AMER CHEMICAL SOC

: 1996

: Bioconjugate Chemistry

: BIOCONJUGATE CHEMISTRY

: BIOCONJUGATE CHEM

: 7

: 2

: 240

: 248

: 9

: 1043-1802

DOI: https://doi.org/10.1021/bc9600067


Synthesis of new non-nucleosidic phosphoramidites 1 and 2 derived from achiral precursors containing two reporter groups or amino functions is described. Among them, only phosphoramidites 1a-c allow multiple derivatization of synthetic oligonucleotides. The usefulness of building blocks 1a-c is demonstrated by introduction of several amino functions or fluorescent dansyl reporters at the 3'- and 5'-termini of synthetic oligonucleotides and their phosphorothioate analogues. It is demonstrated that multilabeled non-nucleosidic tether has no or only minor influence on the hybridization properties of the oligonucleotides.



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