A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Simple nanoparticle-based luminometric method for molecular weight determination of polymeric compounds
Tekijät: Sari Pihlasalo, Maria Virtamo, Nicolas Legrand, Pekka Hänninen, Harri Härmä
Kustantaja: American Chemical Society
Julkaisuvuosi: 2014
Journal: Analytical Chemistry
Tietokannassa oleva lehden nimi: Analytical chemistry
Lehden akronyymi: Anal Chem
Vuosikerta: 86
Numero: 2
Aloitussivu: 1038
Lopetussivu: 1044
Sivujen määrä: 7
ISSN: 1520-6882
eISSN: 1520-6882
DOI: https://doi.org/10.1021/ac401797m
Tiivistelmä
A nanoparticle-based method utilizing time-resolved luminescence resonance energy transfer (TR-LRET) was developed for molecular weight determination. This mix-and-measure nanoparticle method is based on the competitive adsorption between the analyte and the acceptor-labeled protein to donor Eu(III) nanoparticles. The size-dependent adsorption of molecules enables the molecular weight determination of differently sized polymeric compounds down to a concentration level of micrograms per liter. The molecular weight determination from 1 to 10 kDa for polyamino acids and from 0.3 to 70 kDa for polyethylene imines is demonstrated. The simple and cost-effective nanoparticle method as microtiter plate assay format shows great potential for the detection of the changes in molecular weight or for quantification of differently sized molecules in biochemical laboratories and in industrial polymeric processes.
A nanoparticle-based method utilizing time-resolved luminescence resonance energy transfer (TR-LRET) was developed for molecular weight determination. This mix-and-measure nanoparticle method is based on the competitive adsorption between the analyte and the acceptor-labeled protein to donor Eu(III) nanoparticles. The size-dependent adsorption of molecules enables the molecular weight determination of differently sized polymeric compounds down to a concentration level of micrograms per liter. The molecular weight determination from 1 to 10 kDa for polyamino acids and from 0.3 to 70 kDa for polyethylene imines is demonstrated. The simple and cost-effective nanoparticle method as microtiter plate assay format shows great potential for the detection of the changes in molecular weight or for quantification of differently sized molecules in biochemical laboratories and in industrial polymeric processes.
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