A1 Refereed original research article in a scientific journal
An efficient approach for screening minimal PKS genes from Streptomyces
Authors: Metsa-Ketela M, Salo V, Halo L, Hautala A, Hakala J, Mantsala P, Ylihonko K
Publisher: ELSEVIER SCIENCE BV
Publication year: 1999
Journal:: FEMS Microbiology Letters
Journal name in source: FEMS MICROBIOLOGY LETTERS
Journal acronym: FEMS MICROBIOL LETT
Volume: 180
Issue: 1
First page : 1
Last page: 6
Number of pages: 6
ISSN: 0378-1097
Abstract
Degenerated oligonucleotide primers were designed to amplify fragments of ketosynthase genes from polyketide antibiotics producing Streptomyces spp. and bacterial strains enriched from soil samples. Cell lysates were used as templates in amplification, so time-consuming DNA purification was avoided. A phylogenetic tree constructed from the amino acid sequences of the amplified fragments shows a distribution of spore pigments and antibiotics in separate classes. In addition, several different subgroups form within the antibiotics group. Anthracyclines were divided into separate branches according to the starter unit used in biosynthesis. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Degenerated oligonucleotide primers were designed to amplify fragments of ketosynthase genes from polyketide antibiotics producing Streptomyces spp. and bacterial strains enriched from soil samples. Cell lysates were used as templates in amplification, so time-consuming DNA purification was avoided. A phylogenetic tree constructed from the amino acid sequences of the amplified fragments shows a distribution of spore pigments and antibiotics in separate classes. In addition, several different subgroups form within the antibiotics group. Anthracyclines were divided into separate branches according to the starter unit used in biosynthesis. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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