A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs
Tekijät: de Mera RMMF, Li LL, Popinigis A, Cisek K, Tuittila M, Yadav L, Serva A, Courtney MJ
Kustantaja: NATURE PUBLISHING GROUP
Julkaisuvuosi: 2017
Journal: Nature Communications
Tietokannassa oleva lehden nimi: NATURE COMMUNICATIONS
Lehden akronyymi: NAT COMMUN
Artikkelin numero: ARTN 15017
Vuosikerta: 8
Sivujen määrä: 18
ISSN: 2041-1723
eISSN: 2041-1723
DOI: https://doi.org/10.1038/ncomms15017
Verkko-osoite: http://www.nature.com/articles/ncomms15017
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/24961009
Engineering light-sensitive protein regulators has been a tremendous multidisciplinary challenge. Optogenetic regulators of MAPKs, central nodes of cellular regulation, have not previously been described. Here we present OptoJNKi, a light-regulated JNK inhibitor based on the AsLOV2 light-sensor domain using the ubiquitous FMN chromophore. OptoJNKi genetransfer allows optogenetic applications, whereas protein delivery allows optopharmacology. Development of OptoJNKi suggests a design principle for other optically regulated inhibitors. From this, we generate Optop38i, which inhibits p38MAPK in intact illuminated cells. Neurons are known for interpreting temporally-encoded inputs via interplay between ion channels, membrane potential and intracellular calcium. However, the consequences of temporal variation of JNK-regulating trophic inputs, potentially resulting from synaptic activity and reversible cellular protrusions, on downstream targets are unknown. Using OptoJNKi, we reveal maximal regulation of c-Jun transactivation can occur at unexpectedly slow periodicities of inhibition depending on the inhibitor's subcellular location. This provides evidence for resonance in metazoan JNK-signalling circuits.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
