A1 Refereed original research article in a scientific journal
Mass spectrometric (HPLC/ESI-MS/MS) quantification of pyrimido[1,2-a]purin-10(3H)-one, a guanine adduct formed by reaction of malondialdehyde with DNA
Authors: Hakala K, Auriola S, Koivisto A, Lonnberg H
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 1999
Journal:: Journal of Pharmaceutical and Biomedical Analysis
Journal name in source: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Journal acronym: J PHARMACEUT BIOMED
Volume: 21
Issue: 5
First page : 1053
Last page: 1061
Number of pages: 9
ISSN: 0731-7085
DOI: https://doi.org/10.1016/S0731-7085(99)00228-9
Abstract
A high performance liquid chromatography/electrospray ionization - tandem mass spectrometric (HPLC/ESI- MS/ MS) method has been developed for quantification of pyrimido[1,2-a]purin-10(3H)-one adducts from DNA. The method is based on acid-catalyzed cleavage of the adducts from DNA and the use of [2,3a,10-C-13(3)]pyrimido[1,2-a]purin-10(3H)-one as an internal standard in the analysis. For this purpose the latter compound was prepared. Rate constants for the acidcatalyzed cleavage of pyrimido[1,2-a]purin-10(3H)-one from the corresponding 2'-deoxyribonucleoside were determined, and its hydrolytic stability and possible formation by a cross reaction between guanine and [2,3a,10]pyrimido[1,2-a]purin-10(3H)-one were studied. (C) 1999 Elsevier Science B.V. All rights reserved.
A high performance liquid chromatography/electrospray ionization - tandem mass spectrometric (HPLC/ESI- MS/ MS) method has been developed for quantification of pyrimido[1,2-a]purin-10(3H)-one adducts from DNA. The method is based on acid-catalyzed cleavage of the adducts from DNA and the use of [2,3a,10-C-13(3)]pyrimido[1,2-a]purin-10(3H)-one as an internal standard in the analysis. For this purpose the latter compound was prepared. Rate constants for the acidcatalyzed cleavage of pyrimido[1,2-a]purin-10(3H)-one from the corresponding 2'-deoxyribonucleoside were determined, and its hydrolytic stability and possible formation by a cross reaction between guanine and [2,3a,10]pyrimido[1,2-a]purin-10(3H)-one were studied. (C) 1999 Elsevier Science B.V. All rights reserved.
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