A1 Journal article – refereed

RNA-binding protein L1TD1 interacts with LIN28 via RNA and is required for human embryonic stem cell self-renewal and cancer cell proliferation

List of Authors: Elisa Närvä, Nelly Rahkonen, Maheswara Reddy Emani, Riikka Lund, Juha-Pekka Pursiheimo, Juuso Nästi, Reija Autio, Omid Rasool, Konstantin Denessiouk, Harri Lähdesmäki, Anjana Rao, Riitta Lahesmaa

Publisher: Wiley Online Library

Publication year: 2012


Number in series: 3

Volume number: 30

Issue number: 3

Number of pages: 9

ISSN: 1066-5099

DOI: http://dx.doi.org/10.1002/stem.1013

URL: http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291549-4918


Human embryonic stem cells (hESC) have a unique capacity to self-renew and differentiate into all the cell types found in human body. Although the transcriptional regulators of pluripotency are well studied, the role of cytoplasmic regulators is still poorly characterized. Here, we report a new stem cell-specific RNA-binding protein L1TD1 (ECAT11, FLJ10884) required for hESC self-renewal and cancer cell proliferation. Depletion of L1TD1 results in immediate downregulation of OCT4 and NANOG. Furthermore, we demonstrate that OCT4, SOX2, and NANOG all bind to the promoter of L1TD1. Moreover, L1TD1 is highly expressed in seminomas, and depletion of L1TD1 in these cancer cells influences self-renewal and proliferation. We show that L1TD1 colocalizes and interacts with LIN28 via RNA and directly with RNA helicase A (RHA). LIN28 has been reported to regulate translation of OCT4 in complex with RHA. Thus, we hypothesize that L1TD1 is part of the L1TD1-RHA-LIN28 complex that could influence levels of OCT4. Our results strongly suggest that L1TD1 has an important role in the regulation of stemness.

Last updated on 2021-24-06 at 11:44