A1 Refereed original research article in a scientific journal
Determination of total and unbound sufentanil in human plasma by ultrafiltration and LC-MS/MS: Application to clinical pharmacokinetic study
Authors: Saari TI, Fechner J, Ihmsen H, Schuttler J, Jeleazcov C
Publisher: ELSEVIER SCIENCE BV
Publication year: 2012
Journal: Journal of Pharmaceutical and Biomedical Analysis
Journal name in source: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Journal acronym: J PHARMACEUT BIOMED
Volume: 66
First page : 306
Last page: 313
Number of pages: 8
ISSN: 0731-7085
DOI: https://doi.org/10.1016/j.jpba.2012.03.050
Abstract
A sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of sufentanil total and unbound drug concentrations. Unbound drug was separated by an ultrafiltration step before sample preparation. Both the ultrafiltrate and plasma samples were extracted with solid-phase extraction and substituted with deuterated sufentanil used as an internal standard. Separation was performed by gradient elution using UPLC-like system and analysed by MS/MS consisting of an electrospray ionization source. Calibration curves showed linearity in the concentration range of 5-2500 pg/ml for analysis of both total and unbound concentrations of sufentanil. The lower limit of quantification was 5 pg/ml for both total and unbound sufentanil plasma drug concentrations. Intra- and interassay precision and accuracy did not exceed 15%. Method was applied to pharmacokinetic study in patients undergoing coronary artery bypass grafting. (C) 2012 Elsevier B.V. All rights reserved.
A sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of sufentanil total and unbound drug concentrations. Unbound drug was separated by an ultrafiltration step before sample preparation. Both the ultrafiltrate and plasma samples were extracted with solid-phase extraction and substituted with deuterated sufentanil used as an internal standard. Separation was performed by gradient elution using UPLC-like system and analysed by MS/MS consisting of an electrospray ionization source. Calibration curves showed linearity in the concentration range of 5-2500 pg/ml for analysis of both total and unbound concentrations of sufentanil. The lower limit of quantification was 5 pg/ml for both total and unbound sufentanil plasma drug concentrations. Intra- and interassay precision and accuracy did not exceed 15%. Method was applied to pharmacokinetic study in patients undergoing coronary artery bypass grafting. (C) 2012 Elsevier B.V. All rights reserved.
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