Based on a topological approach that studies law, policy and space intertwined, I present an analysis how the renewable energy governance of the European Union (EU) has influenced the development of transport biofuels globally. The instruments of governance regulate the spaces of producing, consuming and trading biofuels. On the other hand, the rapid development of biofuels has created externalities, impacts that are mediated from these well-governed spaces into other locations, which I discuss under the rubric of the dislocated spatiality of biofuels. I use this concept to explicate the shortcomings and challenges that are not tackled by the EU. Moreover, my analysis reveals how the recent biofuel policy of the European Commission is increasingly built on a set of instruments that are grounded through the calculation of greenhouse gas emissions. This re-orientation does not only denote a drastic change in logic of promoting biofuels in the Union but also it shifts the focus from governing actual actors and elements associated with the EU’s biofuel development into quantitative realms construed through models.