A1 Refereed original research article in a scientific journal
Hydrolytic stability of nucleoside phosphotriesters derived from bis(hydroxymethyl)-1,3-dicarbonyl compounds and their congeners: towards a novel pro-drug strategy for antisense oligonucleotides
Authors: Ora M, Maki E, Poijarvi P, Neuvonen K, Oivanan M, Lonnberg H
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2001
Journal:Journal of the Chemical Society, Perkin Transactions 2
Journal name in sourceJOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Journal acronym: J CHEM SOC PERK T 2
Issue: 6
First page : 881
Last page: 885
Number of pages: 5
ISSN: 1472-779X
DOI: https://doi.org/10.1039/b101754n
Abstract
Four different nucleoside phosphodiester protecting groups derived from bis(hydroxymethyl)-1,3-dicarbonyl compounds and their congeners have been prepared and introduced to 5'-O-pivaloylthymidine 3'-(2-methoxyethyl)phosphate and its monothioate analogs. Nucleoside phosphotriesters having either 2,2-bis(ethoxycarbonyl)-3-(4,4'-dimethoxytrityloxy)propyl (1a), 2-cyano-2-methoxycarbonyl-3-(4,4'-dimethoxytrityloxy)propyl (1b), 2,2-bis(cyano)-3-(4,4'-dimethoxytrityloxy)propyl (1c) or 2-acetyl-2-benzoyl-3-(4,4'-dimethoxytrityloxy)propyl (1d) protecting group have been prepared. Additionally were synthesized the O- and S-esterified phosphoromonothioate analogs of 1b. After removal of the dimethoxytrityl groupunder acidic conditions, each of the detritylated protecting groups is readily cleaved from the phosphate/thiophosphate moiety by a reaction suggested to involve a base-catalyzed retro-aldol condensation and following elimination of the phosphodiester from the formed carbanion intermediate. The kinetics of the hydroxide ion-catalyzed cleavage have been studied by HPLC over a pH range 2-7. The half-lives of the cleavage at pH 7 and 25 degreesC vary from 0.3 s (for 1c) to 5500 s (for 1a). The results confirm that these protecting groups have promising chemical properties for use in the development of antisense oligonucleotide pro-drug strategies.
Four different nucleoside phosphodiester protecting groups derived from bis(hydroxymethyl)-1,3-dicarbonyl compounds and their congeners have been prepared and introduced to 5'-O-pivaloylthymidine 3'-(2-methoxyethyl)phosphate and its monothioate analogs. Nucleoside phosphotriesters having either 2,2-bis(ethoxycarbonyl)-3-(4,4'-dimethoxytrityloxy)propyl (1a), 2-cyano-2-methoxycarbonyl-3-(4,4'-dimethoxytrityloxy)propyl (1b), 2,2-bis(cyano)-3-(4,4'-dimethoxytrityloxy)propyl (1c) or 2-acetyl-2-benzoyl-3-(4,4'-dimethoxytrityloxy)propyl (1d) protecting group have been prepared. Additionally were synthesized the O- and S-esterified phosphoromonothioate analogs of 1b. After removal of the dimethoxytrityl groupunder acidic conditions, each of the detritylated protecting groups is readily cleaved from the phosphate/thiophosphate moiety by a reaction suggested to involve a base-catalyzed retro-aldol condensation and following elimination of the phosphodiester from the formed carbanion intermediate. The kinetics of the hydroxide ion-catalyzed cleavage have been studied by HPLC over a pH range 2-7. The half-lives of the cleavage at pH 7 and 25 degreesC vary from 0.3 s (for 1c) to 5500 s (for 1a). The results confirm that these protecting groups have promising chemical properties for use in the development of antisense oligonucleotide pro-drug strategies.
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