A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Study on luminescent ternary EuEDTA complexes with a set of substituted 4-phenylethynyl and 4-aryl pyridine-2,6-dicarboxylic acids
Tekijät: Räsänen Markus, Rosenberg Jaana, Lukkari Jukka, Haapakka Keijo, Kankare Jouko, Takalo Harri
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2017
Journal: Journal of Luminescence
Tietokannassa oleva lehden nimi: JOURNAL OF LUMINESCENCE
Lehden akronyymi: J LUMIN
Vuosikerta: 187
Aloitussivu: 471
Lopetussivu: 478
Sivujen määrä: 8
ISSN: 0022-2313
eISSN: 1872-7883
DOI: https://doi.org/10.1016/j.jlumin.2017.03.061
Tiivistelmä
A set of pyridine-2,6-dicarboxylic acids (dipicolinic acids, DPAD, Lx) were prepared to study the photo physical properties of their ternary complexes with Eu(III) and ethylenediaminetetraacetic acid (EDTA or Y). The stability constant of the formation of EuY-L1 (L1= dipicolinic acid, measured log K-ter =5.38) was determined and found to be comparable to the formation constant of Eu(L1)(3)(logK(3) =5.51). All the studied ternary complexes exhibit two predominant luminescence decay times. The origin of the shorter lifetime was shown to be due to the dissociation of carboxylate group(s) and their replacement by water molecules. The presence of the short-lived component decreases the quantum yields compared those reported for complexes of Eu(Lx)(3). However, the use of ternary complexes of EuY-Lx offers a simple comparison method between different dipicolinic acids (Lx). Eight of the studied ligands Lx form brighter ternary complexes than the currently used ligand in the duplex PCR assay, with quantum yields over 20%, and the most luminescent one has three times higher signal level compared to the currently used ligand. (C) 2017 Elsevier B.V. All rights reserved.
A set of pyridine-2,6-dicarboxylic acids (dipicolinic acids, DPAD, Lx) were prepared to study the photo physical properties of their ternary complexes with Eu(III) and ethylenediaminetetraacetic acid (EDTA or Y). The stability constant of the formation of EuY-L1 (L1= dipicolinic acid, measured log K-ter =5.38) was determined and found to be comparable to the formation constant of Eu(L1)(3)(logK(3) =5.51). All the studied ternary complexes exhibit two predominant luminescence decay times. The origin of the shorter lifetime was shown to be due to the dissociation of carboxylate group(s) and their replacement by water molecules. The presence of the short-lived component decreases the quantum yields compared those reported for complexes of Eu(Lx)(3). However, the use of ternary complexes of EuY-Lx offers a simple comparison method between different dipicolinic acids (Lx). Eight of the studied ligands Lx form brighter ternary complexes than the currently used ligand in the duplex PCR assay, with quantum yields over 20%, and the most luminescent one has three times higher signal level compared to the currently used ligand. (C) 2017 Elsevier B.V. All rights reserved.
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