A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Yersiniophage phi R1-37 is a tailed bacteriophage having a 270 kb DNA genome with thymidine replaced by deoxyuridine
Tekijät: Kiljunen S, Hakala K, Pinta E, Huttunen S, Pluta P, Gador A, Lonnberg H, Skurnik M
Kustantaja: SOC GENERAL MICROBIOLOGY
Julkaisuvuosi: 2005
Lehti:: Microbiology
Tietokannassa oleva lehden nimi: MICROBIOLOGY-SGM
Lehden akronyymi: MICROBIOL-SGM
Vuosikerta: 151
Aloitussivu: 4093
Lopetussivu: 4102
Sivujen määrä: 10
ISSN: 1350-0872
DOI: https://doi.org/10.1099/mic.0.28265-0
Tiivistelmä
Bacteriophage phi R1-37 was isolated based on its ability to infect strain YeO(3)-R1, a virulence-plasmid-cured O antigen-negative derivative of Yersinia enterocolitica serotype O : 3. In this study, the phage receptor was found to be a structure in the outer core hexasaccharide of Y. enterocolitica O : 3 LPS. The phage receptor was present in the outer core of strains of many other Y. enterocolitica serotypes, but also in some Yersinia intermedia strains. Surprisingly, the receptor structure resided in the O antigen of Yersinia pseudotuberculosis O : 9. Electron microscopy demonstrated that phi R1-37 particles have an icosahedral head of 88 nm, a short neck of 10 nm, a long contractile tail of 236 nm, and tail fibres of at least 86 nm. This implies that the phage belongs to the order Caudovirales and the family Myoviridae in the ICTV (International Committee for Taxonomy of Viruses) classification. phi R1-37 was found to have a lytic life cycle, with eclipse and latent periods of 40 and 50 min, respectively, and a burst size of similar to 80 p.f.u. per infected cell. Restriction digestions and PFGE showed that the phi R1-37 genome was dsDNA and similar to 270 kb in size. Enzymically hydrolysed DNA was subjected to HPLC-MS/MS analysis, which demonstrated that the phi R1-37 genome is composed of DNA in which thymidine (T) is > 99 % replaced by deoxyuridine (dU). The only organisms known to have similar DNA are the Bacillus subtilis-specific bacteriophages PBS1 and PBS2. N-terminal amino acid sequences of four major structural proteins did not show any similarity to (viral) protein sequences in databases, indicating that close relatives of phi R1-37 have not yet been characterized. Genes for two of the structural proteins, p24 and p46, were identified from the partially sequenced phi R1-37 genome.
Bacteriophage phi R1-37 was isolated based on its ability to infect strain YeO(3)-R1, a virulence-plasmid-cured O antigen-negative derivative of Yersinia enterocolitica serotype O : 3. In this study, the phage receptor was found to be a structure in the outer core hexasaccharide of Y. enterocolitica O : 3 LPS. The phage receptor was present in the outer core of strains of many other Y. enterocolitica serotypes, but also in some Yersinia intermedia strains. Surprisingly, the receptor structure resided in the O antigen of Yersinia pseudotuberculosis O : 9. Electron microscopy demonstrated that phi R1-37 particles have an icosahedral head of 88 nm, a short neck of 10 nm, a long contractile tail of 236 nm, and tail fibres of at least 86 nm. This implies that the phage belongs to the order Caudovirales and the family Myoviridae in the ICTV (International Committee for Taxonomy of Viruses) classification. phi R1-37 was found to have a lytic life cycle, with eclipse and latent periods of 40 and 50 min, respectively, and a burst size of similar to 80 p.f.u. per infected cell. Restriction digestions and PFGE showed that the phi R1-37 genome was dsDNA and similar to 270 kb in size. Enzymically hydrolysed DNA was subjected to HPLC-MS/MS analysis, which demonstrated that the phi R1-37 genome is composed of DNA in which thymidine (T) is > 99 % replaced by deoxyuridine (dU). The only organisms known to have similar DNA are the Bacillus subtilis-specific bacteriophages PBS1 and PBS2. N-terminal amino acid sequences of four major structural proteins did not show any similarity to (viral) protein sequences in databases, indicating that close relatives of phi R1-37 have not yet been characterized. Genes for two of the structural proteins, p24 and p46, were identified from the partially sequenced phi R1-37 genome.
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