A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Sphingolipids inhibit vimentin-dependent cell migration




Julkaisun tekijät: Hyder CL, Kemppainen K, Isoniemi KO, Imanishi SY, Goto H, Inagaki M, Fazeli E, Eriksson JE, Tornquist K

Kustantaja: COMPANY OF BIOLOGISTS LTD

Julkaisuvuosi: 2015

Journal: Journal of Cell Science

Tietokannassa oleva lehden nimi: JOURNAL OF CELL SCIENCE

Lehden akronyymi: J CELL SCI

Volyymi: 128

Julkaisunumero: 11

Sivujen määrä: 13

ISSN: 0021-9533

eISSN: 1477-9137

DOI: http://dx.doi.org/10.1242/jcs.160341


Tiivistelmä

The sphingolipids, sphingosine 1-phosphate (S1P) and sphingosylphosphorylcholine (SPC), can induce or inhibit cellular migration. The intermediate filament protein vimentin is an inducer of migration and a marker for epithelial-mesenchymal transition. Given that keratin intermediate filaments are regulated by SPC, with consequences for cell motility, we wanted to determine whether vimentin is also regulated by sphingolipid signalling and whether it is a determinant for sphingolipid-mediated functions. In cancer cells where S1P and SPC inhibited migration, we observed that S1P and SPC induced phosphorylation of vimentin on S71, leading to a corresponding reorganization of vimentin filaments. These effects were sphingolipid-signalling-dependent, because inhibition of either the S1P2 receptor (also known as S1PR2) or its downstream effector Rho-associated kinase (ROCK, for which there are two isoforms ROCK1 and ROCK2) nullified the sphingolipid-induced effects on vimentin organization and S71 phosphorylation. Furthermore, the anti-migratory effect of S1P and SPC could be prevented by expressing S71-phosphorylation-deficient vimentin. In addition, we demonstrated, by using wild-type and vimentin-knockout mouse embryonic fibroblasts, that the sphingolipid-mediated inhibition of migration is dependent on vimentin. These results imply that this newly discovered sphingolipid-vimentin signalling axis exerts brake-and-throttle functions in the regulation of cell migration.


Last updated on 2021-24-06 at 11:30