A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Hypoxia exposure and B-type natriuretic peptide release from Langendorff heart of rats
Tekijät: Anttila K, Streng T, Pispa J, Vainio M, Nikinmaa M
Kustantaja: WILEY
Julkaisuvuosi: 2017
Journal: Acta Physiologica
Tietokannassa oleva lehden nimi: ACTA PHYSIOLOGICA
Lehden akronyymi: ACTA PHYSIOL
Vuosikerta: 220
Numero: 1
Aloitussivu: 28
Lopetussivu: 35
Sivujen määrä: 8
ISSN: 1748-1708
eISSN: 1748-1716
DOI: https://doi.org/10.1111/apha.12767
Verkko-osoite: http://onlinelibrary.wiley.com/doi/10.1111/apha.12767/epdf
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/22618378
AimWe studied whether available oxygen without induced mechanical stretch regulates the release of the biologically active B-type natriuretic peptide (BNP) from Langendorff heart.MethodsRat hearts were isolated and perfused with a physiological Krebs-Henseleit solution at a constant hydrostatic pressure in Langendorff set-up. The basal O-2 level of perfusate (24.40.04mgL(-1)) was gradually lowered to 3.00.01mgL(-1) over 20min using N-2 gas (n=7). BNP and O-2 level were measured from coronary flow. During control perfusions (n=5), the O-2 concentration was kept at 26.6 +/- 0.3mgL(-1).ResultsA low oxygen concentration in the perfusate was associated with a significant increase in BNP release (F=40.4, P<0.001). Heart rate decreased when the oxygen concentration in the perfusate reached 9.1 +/- 0.02mgL(-1) and continued to fall in lower oxygen concentrations (F=14.8, P<0.001). There was also a significant but inverse correlation between BNP and oxygen in the coronary flow (R-2=0.27, P<0.001).ConclusionIn the spontaneously beating Langendorff rat heart, a decreasing concentration of oxygen in the ingoing perfusion increased the secretion of BNP. The effect of oxygen was independent of mechanical stretch of the heart as it occurred even when the heart rate decreased but the pressure conditions remained constant. The difference in the oxygen capacitance of blood and Krebs-Henseleit solution appears to be a major factor affecting secretion of BNP, which is correlated with the oxygen tension of myocardial cells and affected both by the oxygen concentration and capacitance of solution perfusing the heart and by the coronary flow.
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