A1 Refereed original research article in a scientific journal
Stressor-dependent regulation of the heat shock response in zebrafish, Danio rerio
Authors: Airaksinen S, Rabergh CMI, Lahti A, Kaatrasalo A, Sistonen L, Nikinmaa M
Publisher: ELSEVIER SCIENCE INC
Publication year: 2003
Journal: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology
Journal name in source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Journal acronym: COMP BIOCHEM PHYS A
Volume: 134
Issue: 4
First page : 839
Last page: 846
Number of pages: 8
ISSN: 1095-6433
DOI: https://doi.org/10.1016/S1095-6433(03)00033-3
Abstract
Heat shock transcription factors (HSFs) regulate expression of heat shock proteins (Hsps). We have previously shown that in zebrafish a unique isoform, zHSF1b, disappears concomitant with heat shock-induced Hsp70 expression. To characterize the role of zHSF1a and zHSF1b isoforms in the, regulation of the stress response in vivo, we have carried out cadmium (10-100 muM) and copper (10-30 muM) exposures in order to specify whether the disappearance of HSF1b is specific for heat stress. After 4-h metal exposures we analyzed the expression of hsp70, zHSF1a, zHSF1b and metallothionein (MT) by reverse transcriptase polymerase chain reaction in zebrafish liver, gonads and gills. Although cadmium is a known inducer of Hsps, it did not affect hsp70 expression significantly in the studied tissues. Induction of hsp70 was observed upon copper exposure in liver and gonads, but not in gills. Neither metal affected the zHSF1a/b ratio. Both cadmium and copper exposure caused upregulation of MT, regulator of metal homeostasis and detoxification, confirming that the tissues were subjected to metal loads. Thus, hsp70 appears to be more weakly induced upon metal exposure than in response to heat shock and HSF1 isoforms may participate in stressor-specific regulation of hsp70. (C) 2003 Elsevier Science Inc. All rights reserved.
Heat shock transcription factors (HSFs) regulate expression of heat shock proteins (Hsps). We have previously shown that in zebrafish a unique isoform, zHSF1b, disappears concomitant with heat shock-induced Hsp70 expression. To characterize the role of zHSF1a and zHSF1b isoforms in the, regulation of the stress response in vivo, we have carried out cadmium (10-100 muM) and copper (10-30 muM) exposures in order to specify whether the disappearance of HSF1b is specific for heat stress. After 4-h metal exposures we analyzed the expression of hsp70, zHSF1a, zHSF1b and metallothionein (MT) by reverse transcriptase polymerase chain reaction in zebrafish liver, gonads and gills. Although cadmium is a known inducer of Hsps, it did not affect hsp70 expression significantly in the studied tissues. Induction of hsp70 was observed upon copper exposure in liver and gonads, but not in gills. Neither metal affected the zHSF1a/b ratio. Both cadmium and copper exposure caused upregulation of MT, regulator of metal homeostasis and detoxification, confirming that the tissues were subjected to metal loads. Thus, hsp70 appears to be more weakly induced upon metal exposure than in response to heat shock and HSF1 isoforms may participate in stressor-specific regulation of hsp70. (C) 2003 Elsevier Science Inc. All rights reserved.
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