A1 Refereed original research article in a scientific journal
Effects of phosphate and light on growth of and bioactive peptide production by the cyanobacterium Anabaena strain 90 and its anabaenopeptilide mutant
Authors: Repka S, Koivula M, Harjunpa V, Rouhiainen L, Sivonen K
Publisher: AMER SOC MICROBIOLOGY
Publication year: 2004
Journal:: Applied and Environmental Microbiology
Journal name in source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Journal acronym: APPL ENVIRON MICROB
Volume: 70
Issue: 8
First page : 4551
Last page: 4560
Number of pages: 10
ISSN: 0099-2240
DOI: https://doi.org/10.1128/AEM.70.8.4551-4560.2004
Abstract
Cyanobacteria synthesize several types of bioactive secondary metabolites. Anabaena strain 90 produces three types of bioactive peptides, microcystins (inhibitors of protein phosphatases 1 and 2A), anabaenopeptilides, and anabaenopeptins (serine protease inhibitors). To investigate the role of the anabaenopeptilides in Anabaena, wild-type strain 90 (WT) and its anabaenopeptilide deficient mutant (MU) were cultured with various light and phosphate levels to evaluate the effects and coeffects of these growth factors on the concentrations of the three classes of peptides and the growth characteristics. WT and MU grew in comparable ways under the different growth conditions. The total peptide concentration in WT was significantly higher than that in MU (2.5 and 1.4 mug/mg [dry weight], respectively). Interestingly, the average concentration of anabaenopeptins was significantly higher in MU than in WT (0.59 and 0.24 mug/mg [dry weight], respectively). The concentration of microcystins was slightly but not statistically significantly higher in MU than in WT (1.0 and 0.86 mug/mg [dry weight], respectively). In WT, the highest peptide concentrations were usually found after 13 days in cultures grown at medium light intensities (23 mumol m(-2) s(-1)) and with the highest phosphate concentrations (2,600 mug liter(-1)). In MU, the highest peptide concentrations were found in 13-day-old cultures grown at medium light intensities (23 mumol m(-2) s(-1)) and with phosphate concentrations greater than 100 mug liter(-1). The higher concentrations of anabaenopeptins in MU may compensate for the absence of anabaenopeptilides. These findings clearly indicate that these compounds may have some linked function in the producer organism, the nature of which remains to be discovered.
Cyanobacteria synthesize several types of bioactive secondary metabolites. Anabaena strain 90 produces three types of bioactive peptides, microcystins (inhibitors of protein phosphatases 1 and 2A), anabaenopeptilides, and anabaenopeptins (serine protease inhibitors). To investigate the role of the anabaenopeptilides in Anabaena, wild-type strain 90 (WT) and its anabaenopeptilide deficient mutant (MU) were cultured with various light and phosphate levels to evaluate the effects and coeffects of these growth factors on the concentrations of the three classes of peptides and the growth characteristics. WT and MU grew in comparable ways under the different growth conditions. The total peptide concentration in WT was significantly higher than that in MU (2.5 and 1.4 mug/mg [dry weight], respectively). Interestingly, the average concentration of anabaenopeptins was significantly higher in MU than in WT (0.59 and 0.24 mug/mg [dry weight], respectively). The concentration of microcystins was slightly but not statistically significantly higher in MU than in WT (1.0 and 0.86 mug/mg [dry weight], respectively). In WT, the highest peptide concentrations were usually found after 13 days in cultures grown at medium light intensities (23 mumol m(-2) s(-1)) and with the highest phosphate concentrations (2,600 mug liter(-1)). In MU, the highest peptide concentrations were found in 13-day-old cultures grown at medium light intensities (23 mumol m(-2) s(-1)) and with phosphate concentrations greater than 100 mug liter(-1). The higher concentrations of anabaenopeptins in MU may compensate for the absence of anabaenopeptilides. These findings clearly indicate that these compounds may have some linked function in the producer organism, the nature of which remains to be discovered.
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