The time course of L-gulono-gamma-lactone oxidase expression during the larval ontogeny of fish (Acipenser persicus)



: Akbarzadeh A, Haghbeen K, Farahmand H, Nematollahi MA, Nikinmaa M

Publisher: TAYLOR & FRANCIS LTD

: London

: 2013

: Marine and Freshwater Behaviour and Physiology

: MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY

: MAR FRESHW BEHAV PHY

: 5

: 45

: 5

: 349

: 355

: 7

: 1023-6244

DOI: https://doi.org/10.1080/10236244.2013.768002


In our previous study, for the first time in any fish species, the mRNA expression of L-Gulono--lactone oxidase (GULO) was investigated during early development of Persian sturgeon, Acipenser persicus, and it was demonstrated that GULO mRNA was expressed throughout the development of Persian sturgeon. Accordingly, it was suggested that the initial production of GULO and probably ascorbate might occur before hatching during the embryonic stage. To evaluate the correspondence between transcript and protein level, GULO activity of Persian sturgeon during ontogenetic development of embryos and larvae was investigated. The GULO activity was detected in all time-points of the development of Persian sturgeon from embryos to the late larval stage indicating the start of ascorbate synthesis before hatching. However, there was a marked difference in the mRNA/protein activity ratio at different times. As the GULO mRNA and protein production peaks were observed during a time course from embryonic to early larval development before the start of the exogenous feeding, it can be concluded that the vitamin C requirement must initially be fulfilled by endogenous formation.



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