A1 Refereed original research article in a scientific journal

The cleavage of phosphodiester bonds within small RNA bulges in the presence and absence of metal ion catalysts




AuthorsKaukinen U, Bielecki L, Mikkola S, Adamiak RW, Lonnberg H

PublisherROYAL SOC CHEMISTRY

Publication year2001

JournalJournal of the Chemical Society, Perkin Transactions 2

Journal name in sourceJOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2

Journal acronymJ CHEM SOC PERK T 2

Issue7

First page 1024

Last page1031

Number of pages8

ISSN1472-779X

DOIhttps://doi.org/10.1039/b102519h(external)


Abstract
This work studies the effect of RNA structure on the reactivity of RNA phosphodiester bonds. The aim of the study is to evaluate those structural parameters that affect reactivity to support the development of sequence-specific RNA cleaving agents. The cleavage of phosphodiester bonds within one- to five-nucleotide bulges has been studied in buffer solutions (pH 8.4), and in the presence of Zn2+ aqua ion (pH 6.4) or its 1,5,9-triazacyclododecane complex (pH 7.4). Molecular modelling has been applied to study the structure of the substrates. The basis of the reactivity and the catalytic potential of metal ion-based cleaving agents are discussed.


Research Areas



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