A1 Refereed original research article in a scientific journal
The cleavage of phosphodiester bonds within small RNA bulges in the presence and absence of metal ion catalysts
Authors: Kaukinen U, Bielecki L, Mikkola S, Adamiak RW, Lonnberg H
Publisher: ROYAL SOC CHEMISTRY
Publication year: 2001
Journal: Journal of the Chemical Society, Perkin Transactions 2
Journal name in source: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Journal acronym: J CHEM SOC PERK T 2
Issue: 7
First page : 1024
Last page: 1031
Number of pages: 8
ISSN: 1472-779X
DOI: https://doi.org/10.1039/b102519h(external)
Abstract
This work studies the effect of RNA structure on the reactivity of RNA phosphodiester bonds. The aim of the study is to evaluate those structural parameters that affect reactivity to support the development of sequence-specific RNA cleaving agents. The cleavage of phosphodiester bonds within one- to five-nucleotide bulges has been studied in buffer solutions (pH 8.4), and in the presence of Zn2+ aqua ion (pH 6.4) or its 1,5,9-triazacyclododecane complex (pH 7.4). Molecular modelling has been applied to study the structure of the substrates. The basis of the reactivity and the catalytic potential of metal ion-based cleaving agents are discussed.
This work studies the effect of RNA structure on the reactivity of RNA phosphodiester bonds. The aim of the study is to evaluate those structural parameters that affect reactivity to support the development of sequence-specific RNA cleaving agents. The cleavage of phosphodiester bonds within one- to five-nucleotide bulges has been studied in buffer solutions (pH 8.4), and in the presence of Zn2+ aqua ion (pH 6.4) or its 1,5,9-triazacyclododecane complex (pH 7.4). Molecular modelling has been applied to study the structure of the substrates. The basis of the reactivity and the catalytic potential of metal ion-based cleaving agents are discussed.