A mixed-phase immunoassay based on simultaneous binding of fluorescently tagged and PNA-conjugated peptide epitopes on antibodies: Quantification on PNA-coated microparticles

: Ketomaki K, Lonnberg H

Publisher: AMER CHEMICAL SOC

: 2006

: Bioconjugate Chemistry

: BIOCONJUGATE CHEMISTRY

: BIOCONJUGATE CHEM

: 17

: 4

: 1063

: 1068

: 6

: 1043-1802

DOI: https://doi.org/10.1021/bc0600315

A mixed-phase immunoassay based on simultaneous binding of an antibody to its fluorescently tagged peptide epitope and a PNA conjugate of the same peptide has been developed. As a fluorescent marker, a europium(III) chelate allowing time-resolved measurement from a single particle has been employed. The ternary complex formed in solution is immobilized by Watson-Crick base-pairing to a microparticle bearing a PNA sequence complementary to that present in the complex. The concentration of the antibody in the sample may then be determined by a single particle measurement. Accordingly, different antibodies may in principle be addressed by sequence-specific hybridization to different categorized microparticles.

Chemical sciences