A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Association of MBL2, TLR1, TLR2 and TLR6 Polymorphisms With Production of IFN-γ and IL-12 in BCG Osteitis Survivors R1
Tekijät: Poyhonen L, Kroger L, Huhtala H, Makinen J, Nuolivirta K, Mertsola J, He QS, Korppi M
Kustantaja: LIPPINCOTT WILLIAMS & WILKINS
Julkaisuvuosi: 2017
Journal: Pediatric Infectious Disease Journal
Tietokannassa oleva lehden nimi: PEDIATRIC INFECTIOUS DISEASE JOURNAL
Lehden akronyymi: PEDIATR INFECT DIS J
Vuosikerta: 36
Numero: 2
Aloitussivu: 135
Lopetussivu: 139
Sivujen määrä: 5
ISSN: 0891-3668
eISSN: 1532-0987
DOI: https://doi.org/10.1097/INF.0000000000001375
Tiivistelmä
Background: Interferon-gamma (IFN-gamma) is a key cytokine in defense against mycobacteria, including Bacillus Calmette-Guerin (BCG). Mannose- binding lectin (MBL) and toll-like receptors (TLRs) are pattern-recognizing molecules of innate immunity. The aim of the present study was to investigate the relationship between polymorphisms in MBL, TLR1, TLR2 and TLR6 encoding genes and stimulated IFN-gamma and interleukin-12 (IL-12) ex vivo production in BCG osteitis survivors.Methods: Data on single nucleotide polymorphisms in the MBL2 gene and TLR1, TLR2 and TLR6 genes were available from 132 former BCG osteitis patients, and data on ex vivo IFN-gamma and IL-12 production were available from 115 and 118 patients, respectively. The present study is a secondary analysis of these available data. In an earlier study, we were able to characterize low IFN-gamma and low IL-12 producers after BCG+ IL-12 or BCG+ IFN gamma. stimulations, respectively.Results: Three patients had the homozygous variant MBL2 genotype, and one of them was a low IFN-gamma producer (both concentration and response <5th percentile). The heterozygous variant MBL2 genotype showed no association with IFN-gamma or IL-12 production. The TLR2 variant genotype was present in 14 subjects; 28.6% of them were low IFN-gamma producers versus 7.8% of those 103 with the TLR2 wild genotype (P = 0.037). TLR1 or TLR6 polymorphisms had no significant associations with stimulated ex vivo IFN-gamma or IL-12 production.Conclusions: Preliminary evidence was found that variant genotypes of the MBL2 gene (if homozygous) and variant genotypes of the TLR2 gene (only heterozygotes present) are associated with low IFN-gamma production.
Background: Interferon-gamma (IFN-gamma) is a key cytokine in defense against mycobacteria, including Bacillus Calmette-Guerin (BCG). Mannose- binding lectin (MBL) and toll-like receptors (TLRs) are pattern-recognizing molecules of innate immunity. The aim of the present study was to investigate the relationship between polymorphisms in MBL, TLR1, TLR2 and TLR6 encoding genes and stimulated IFN-gamma and interleukin-12 (IL-12) ex vivo production in BCG osteitis survivors.Methods: Data on single nucleotide polymorphisms in the MBL2 gene and TLR1, TLR2 and TLR6 genes were available from 132 former BCG osteitis patients, and data on ex vivo IFN-gamma and IL-12 production were available from 115 and 118 patients, respectively. The present study is a secondary analysis of these available data. In an earlier study, we were able to characterize low IFN-gamma and low IL-12 producers after BCG+ IL-12 or BCG+ IFN gamma. stimulations, respectively.Results: Three patients had the homozygous variant MBL2 genotype, and one of them was a low IFN-gamma producer (both concentration and response <5th percentile). The heterozygous variant MBL2 genotype showed no association with IFN-gamma or IL-12 production. The TLR2 variant genotype was present in 14 subjects; 28.6% of them were low IFN-gamma producers versus 7.8% of those 103 with the TLR2 wild genotype (P = 0.037). TLR1 or TLR6 polymorphisms had no significant associations with stimulated ex vivo IFN-gamma or IL-12 production.Conclusions: Preliminary evidence was found that variant genotypes of the MBL2 gene (if homozygous) and variant genotypes of the TLR2 gene (only heterozygotes present) are associated with low IFN-gamma production.
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