A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Tocopherols, tocotrienols and fatty acids as indicators of natural ageing in Pinus sylvestris seeds
Tekijät: Tammela P, Salo-Vaananen P, Laakso I, Hopia A, Vuorela H, Nygren M
Kustantaja: TAYLOR & FRANCIS AS
Julkaisuvuosi: 2005
Journal: Scandinavian Journal of Forest Research
Tietokannassa oleva lehden nimi: SCANDINAVIAN JOURNAL OF FOREST RESEARCH
Lehden akronyymi: SCAND J FOREST RES
Vuosikerta: 20
Numero: 5
Aloitussivu: 378
Lopetussivu: 384
Sivujen määrä: 7
ISSN: 0282-7581
DOI: https://doi.org/10.1080/02827580500292063
Tiivistelmä
During long-term storage, Scots pine ( Pinus sylvestris L.) seeds are susceptible to ageing, leading to declined vigour and poor germinability. To characterize biochemical changes associated with this ageing, vitamin E and fatty acid compositions of Scots pine seeds stored for 3-31 years were analysed. The seeds contained seven homologues of the vitamin E family: alpha-, beta-, delta- and gamma-tocopherols (T) and alpha-, delta- and gamma-tocotrienol (T3). The major tocopherol was gamma-T, constituting on average 62.1% of total vitamin E. Total vitamin E concentration was markedly lower in older seeds, e. g. 21-year-old seeds contained 52% less vitamin E than the reference for high-quality seed. The a- T concentration was affected the most by seed age, whereas the gamma-T concentration decreased more gradually and the concentrations of the other E vitamers seemed to remain relatively constant during natural ageing. Ageing also induced changes in the fatty acid composition of the seeds, e. g. a 29.6% reduction in the unsaturation/saturation ratio. These biochemical changes were associated with decreased germinability and increased electrolyte leakage.
During long-term storage, Scots pine ( Pinus sylvestris L.) seeds are susceptible to ageing, leading to declined vigour and poor germinability. To characterize biochemical changes associated with this ageing, vitamin E and fatty acid compositions of Scots pine seeds stored for 3-31 years were analysed. The seeds contained seven homologues of the vitamin E family: alpha-, beta-, delta- and gamma-tocopherols (T) and alpha-, delta- and gamma-tocotrienol (T3). The major tocopherol was gamma-T, constituting on average 62.1% of total vitamin E. Total vitamin E concentration was markedly lower in older seeds, e. g. 21-year-old seeds contained 52% less vitamin E than the reference for high-quality seed. The a- T concentration was affected the most by seed age, whereas the gamma-T concentration decreased more gradually and the concentrations of the other E vitamers seemed to remain relatively constant during natural ageing. Ageing also induced changes in the fatty acid composition of the seeds, e. g. a 29.6% reduction in the unsaturation/saturation ratio. These biochemical changes were associated with decreased germinability and increased electrolyte leakage.
Turun yliopiston Tutkimustietojärjestelmän portaali