A2 Refereed review article in a scientific journal
G Protein-Coupled Receptor Transactivation – From Molecules to Mice
Subtitle: From Molecules to Mice
Authors: Jonas Kim C, Rivero-Müller Adolfo, Huhtaniemi Ilpo T, Hanyaloglu Aylin C
Publication year: 2013
Journal: Methods in Cell Biology
Volume: 117
First page : 433
Last page: 450
Number of pages: 18
ISBN: 978-0-12-408143-7
ISSN: 0091-679X
DOI: https://doi.org/10.1016/B978-0-12-408143-7.00023-2
Abstract
G protein-coupled receptors (GPCRs) mediate a diverse range of physiological functions via activation of complex signaling systems. Organization of GPCRs in to dimers and oligomers provides a mechanism for both signal diversity and specificity in cellular responses, yet our understanding of the physiological significance of dimerization, particularly homodimerization, has not been forthcoming. This chapter will describe how we have investigated the physiological importance of GPCR homodimerization, using the luteinizing hormone/chorionic gonadotropin receptor as a model GPCR. Using transactivation as a mode of assessing receptor dimerization, we describe our cellular system and functional assays for assessment of transactivation in vitro and detail our strategy for generating a mouse model to assess GPCR transactivation in vivo.
G protein-coupled receptors (GPCRs) mediate a diverse range of physiological functions via activation of complex signaling systems. Organization of GPCRs in to dimers and oligomers provides a mechanism for both signal diversity and specificity in cellular responses, yet our understanding of the physiological significance of dimerization, particularly homodimerization, has not been forthcoming. This chapter will describe how we have investigated the physiological importance of GPCR homodimerization, using the luteinizing hormone/chorionic gonadotropin receptor as a model GPCR. Using transactivation as a mode of assessing receptor dimerization, we describe our cellular system and functional assays for assessment of transactivation in vitro and detail our strategy for generating a mouse model to assess GPCR transactivation in vivo.
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