A1 Refereed original research article in a scientific journal
Intracellular Degradation of Multilabeled Poly(Ethylene imine)-Mesoporous Silica-Silica Nanoparticles: Implications for Drug Release
Authors: Bergman L, Kankaanpaa P, Tiitta S, Duchanoy A, Li L, Heino J, Linden M
Publisher: AMER CHEMICAL SOC
Publication year: 2013
Journal: Molecular Pharmaceutics
Journal name in source: MOLECULAR PHARMACEUTICS
Journal acronym: MOL PHARMACEUT
Number in series: 5
Volume: 10
Issue: 5
First page : 1795
Last page: 1803
Number of pages: 9
ISSN: 1543-8384
DOI: https://doi.org/10.1021/mp3005879(external)
Abstract
Mesoporous silica nanoparticles, MSNs, have emerged as an interesting carrier for drugs in vitro and in vivo. The particles are typically used in a surface functionalized form, where functional silanes or other covalently linked surface functions are used to provide anchoring sites for additional functionalities like targeting groups, imaging agents, and drugs. Here, we report results related to extra- and intracellular degradation of silica nanoparticles using multilabeled nonporous silica core-mesoporous silica shell-surface hyperbranched poly(ethylene imine) shell nanoparticles as model particles. Different fluorophores have been selectively covalently linked to different regions of the particles in order to study the particle degradation in detail under in vitro conditions in human SAOS-2 cells. A novel, quantitative method for nanoparticle degradation evaluation based on confocal fluorescence microscopy is applied. Our results suggest that the core-shell-shell MSNs degrade at a higher rate inside cells as compared to outside cells, which is of high importance for further application of this class of drug carriers.
Mesoporous silica nanoparticles, MSNs, have emerged as an interesting carrier for drugs in vitro and in vivo. The particles are typically used in a surface functionalized form, where functional silanes or other covalently linked surface functions are used to provide anchoring sites for additional functionalities like targeting groups, imaging agents, and drugs. Here, we report results related to extra- and intracellular degradation of silica nanoparticles using multilabeled nonporous silica core-mesoporous silica shell-surface hyperbranched poly(ethylene imine) shell nanoparticles as model particles. Different fluorophores have been selectively covalently linked to different regions of the particles in order to study the particle degradation in detail under in vitro conditions in human SAOS-2 cells. A novel, quantitative method for nanoparticle degradation evaluation based on confocal fluorescence microscopy is applied. Our results suggest that the core-shell-shell MSNs degrade at a higher rate inside cells as compared to outside cells, which is of high importance for further application of this class of drug carriers.
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