Blood protein measurements are used frequently
in the clinic in the assessment of patient health. Nevertheless, there remains
the need for new biomarkers with better diagnostic specificities. With the
advent of improved technology for bioanalysis and the growth of biobanks including
collections from specific disease-risk cohorts, the plasma proteome has
remained a target of proteomics research towards the characterization of
disease related biomarkers. The following protocol presents a workflow for serum/plasma
proteomics including details of sample preparation both with and without immunoaffinity
depletion of the most abundant plasma proteins, and methodology for selected
reaction monitoring mass spectrometry validation.