A1 Refereed original research article in a scientific journal
Aptamer-PEG-modified Fe3O4@Mn as a novel T1-and T2-dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells
Authors: Haitao Zhu, Lirong Zhang, Yanfang Liu, Yuepeng Zhou, Kang Wang, Xiaodong Xie, Lian Song, Dongqing Wang, Chunlei Han, Qiuyun Chen
Publisher: NATURE PUBLISHING GROUP
Publication year: 2016
Journal:: Scientific Reports
Journal name in source: SCIENTIFIC REPORTS
Journal acronym: SCI REP-UK
Article number: 39245
Volume: 6
Number of pages: 12
ISSN: 2045-2322
DOI: https://doi.org/10.1038/srep39245
Abstract
Hypoxia-induced cancer stem cells have been known to be involved in tumour metastasis, resistance to chemo/radio therapy and tumour recurrence. Magnetic Resonance Imaging is a widely used imaging tool for cancers in clinics and research. To develop T1-positive and T2-negative dual mode MRI agents for more comprehensive and accurate diagnostic information under hypoxic conditions, a hypoxia inducible factor-1 alpha based aptamer and Mn(II)-modified nanoparticles D-Fe3O4@ PMn were synthesized and characterized. In vitro and in vivo studies show that D-Fe3O4@PMn NPs are biocompatible and less cytotoxic and can produce significant contrast enhancement in T1-and T2-weighted MR imaging. Furthermore, the D-Fe3O4@PMn NPs enable targeted dual-contrast T1-and T2-weighted MR imaging of cancer cells expressing high levels of HIF-1 alpha and cancer stem cell-related proteins under hypoxic condition. In conclusion, NPs with HIF-1 alpha and Mn(II) are promising diagnostic agents for dual-mode T1 and T2 imaging by targeting cancer stem cells as they are non-toxic and biocompatible.
Hypoxia-induced cancer stem cells have been known to be involved in tumour metastasis, resistance to chemo/radio therapy and tumour recurrence. Magnetic Resonance Imaging is a widely used imaging tool for cancers in clinics and research. To develop T1-positive and T2-negative dual mode MRI agents for more comprehensive and accurate diagnostic information under hypoxic conditions, a hypoxia inducible factor-1 alpha based aptamer and Mn(II)-modified nanoparticles D-Fe3O4@ PMn were synthesized and characterized. In vitro and in vivo studies show that D-Fe3O4@PMn NPs are biocompatible and less cytotoxic and can produce significant contrast enhancement in T1-and T2-weighted MR imaging. Furthermore, the D-Fe3O4@PMn NPs enable targeted dual-contrast T1-and T2-weighted MR imaging of cancer cells expressing high levels of HIF-1 alpha and cancer stem cell-related proteins under hypoxic condition. In conclusion, NPs with HIF-1 alpha and Mn(II) are promising diagnostic agents for dual-mode T1 and T2 imaging by targeting cancer stem cells as they are non-toxic and biocompatible.
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