A1 Refereed original research article in a scientific journal
Quantitative analysis of polymeric proanthocyanidins in birch leaves with normal-phase HPLC
Authors: Karonen M, Ossipov V, Sinkkonen J, Loponen J, Haukioja E, Pihlaja K
Publisher: JOHN WILEY & SONS LTD
Publication year: 2006
Journal:Phytochemical Analysis
Journal name in sourcePHYTOCHEMICAL ANALYSIS
Journal acronym: PHYTOCHEM ANALYSIS
Volume: 17
Issue: 3
First page : 149
Last page: 156
Number of pages: 8
ISSN: 0958-0344
DOI: https://doi.org/10.1002/pca.898
Abstract
The proanthocyanidin composition and content in the leaves of nine birch species (Betula albosinensis, B. ermanii B. maximowicziana, B. nana, B. Papyrifiera. B. pendula, B. platyphylla, B. pubescens, and B. pubescens ssp. czerepanovii) were studied with different methods including colorimetric assay, HPLC coupled with PAD or ESI/MS and NMR. Total proanthocyanidin content was determined using the acid butanol assay. A normal phase-HPLC method was applied for the analysis of polymeric proanthocyanidins. The content of polymeric proanthocyanidins was estimated from a late eluting peak in the chromatogram. With this HPLC method, quantitative analysis of polymeric proanthocyanidins could be performed directly from leaf extracts: no additional purification or preparation steps were required. It was shown that birch leaves contained mainly polymeric proanthocyanidins with a degree of polymerisation greater than 10. Total proanthocyanidin content (expressed as dry weight) was found to vary from 44mg/g (B. papyrifera) to 145mg/g (B. nana), and polymeric proanthocyarridin content from 39mg/g (B. pendula) to 119 mg/g (B. nana). Copyright (c) 2006 John Wiley & Sons, Ltd.
The proanthocyanidin composition and content in the leaves of nine birch species (Betula albosinensis, B. ermanii B. maximowicziana, B. nana, B. Papyrifiera. B. pendula, B. platyphylla, B. pubescens, and B. pubescens ssp. czerepanovii) were studied with different methods including colorimetric assay, HPLC coupled with PAD or ESI/MS and NMR. Total proanthocyanidin content was determined using the acid butanol assay. A normal phase-HPLC method was applied for the analysis of polymeric proanthocyanidins. The content of polymeric proanthocyanidins was estimated from a late eluting peak in the chromatogram. With this HPLC method, quantitative analysis of polymeric proanthocyanidins could be performed directly from leaf extracts: no additional purification or preparation steps were required. It was shown that birch leaves contained mainly polymeric proanthocyanidins with a degree of polymerisation greater than 10. Total proanthocyanidin content (expressed as dry weight) was found to vary from 44mg/g (B. papyrifera) to 145mg/g (B. nana), and polymeric proanthocyarridin content from 39mg/g (B. pendula) to 119 mg/g (B. nana). Copyright (c) 2006 John Wiley & Sons, Ltd.
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