A1 Refereed original research article in a scientific journal
TIME-RESOLVED LUMINESCENCE DETECTION OF EUROPIUM(III) CHELATES IN CAPILLARY ELECTROPHORESIS
Authors: LATVA M, ALAKLEME T, BJENNES H, KANKARE J, HAAPAKKA K
Publisher: ROYAL SOC CHEMISTRY
Publication year: 1995
Journal: Analyst
Journal name in source: ANALYST
Journal acronym: ANALYST
Volume: 120
Issue: 2
First page : 367
Last page: 372
Number of pages: 6
ISSN: 0003-2654
DOI: https://doi.org/10.1039/an9952000367
Abstract
An instrumentally simple on-column time-resolved luminescence capillary electrophoresis (CE) detector for determining and characterizing migrated europium(III) chelates on the basis of their long-lived D-5(0) --> F-7-multiplet transitions has been constructed. In addition to the background fluorescence arising from the cell walls, this time-resolved luminescence method of measurement also allows an efficient discrimination of the analyte emission against the short-lived background fluorescence caused by possible fluorescent impurities in the electrophoresis buffer; this should provide a feasible means of constructing a CE detector for the trace analysis of europium(III) chelates and also of samples of biological interest labelled with europium(III) chelates, If supplied additionally with a device for determining the excited-state lifetimes of separated europium(III) chelates, this time-resolved luminescence detector should make possible the peak identification of migrated europium(III) chelates.
An instrumentally simple on-column time-resolved luminescence capillary electrophoresis (CE) detector for determining and characterizing migrated europium(III) chelates on the basis of their long-lived D-5(0) --> F-7-multiplet transitions has been constructed. In addition to the background fluorescence arising from the cell walls, this time-resolved luminescence method of measurement also allows an efficient discrimination of the analyte emission against the short-lived background fluorescence caused by possible fluorescent impurities in the electrophoresis buffer; this should provide a feasible means of constructing a CE detector for the trace analysis of europium(III) chelates and also of samples of biological interest labelled with europium(III) chelates, If supplied additionally with a device for determining the excited-state lifetimes of separated europium(III) chelates, this time-resolved luminescence detector should make possible the peak identification of migrated europium(III) chelates.
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