Mechanism and analytical applicability of luminol specific extrinsic lyoluminescence of UV-irradiated potassium peroxodisulfate



: Matachescu C, Kulmala S, Ala-Kleme T, Joela H

: 1997

 Analytical Chemistry

: ANALYTICAL CHEMISTRY

: ANAL CHEM

: 69

: 16

: 3385

: 3390

: 6

: 0003-2700

DOI: https://doi.org/10.1021/ac9611733


Luminol shows strong chemiluminescence with an emission maximum at similar to 430 nm in the presence of sulfate radicals. Sulfate radicals were produced by the dissolution of UV-irradiated potassium peroxodisulfate powder in aqueous luminol solutions. The UV irradiation at 6.7 eV produces a solid solution of sulfate radical in potassium peroxodisulfate by rupturing -O-O- bonds, as in solution, but now the solid solution is stable in a time scale of years in dryness. In the present system, luminol chemiluminescence is produced via several parallel pathways having a common triggering step, one-electron oxidation of luminol monoanion by sulfate or hydroxyl radical. Present chemiluminescence allows sensitive luminol detection from picomolar to micromolar level with a linear response over 5 orders of magnitude, after which luminescence is too strong for single-photon counting. The high sensitivity of luminol detection allows us to propose extrinsic lyoluminescence of potassium peroxodisulfate as a new and simple method for detection step of bioaffinity assays using luminol or isoluminol derivatives as label compounds.



Last updated on 26/11/2024 07:05:03 PM