A1 Refereed original research article in a scientific journal
Mechanism and analytical applicability of luminol specific extrinsic lyoluminescence of UV-irradiated potassium peroxodisulfate
Authors: Matachescu C, Kulmala S, Ala-Kleme T, Joela H
Publisher: AMER CHEMICAL SOC
Publication year: 1997
Journal:: Analytical Chemistry
Journal name in source: ANALYTICAL CHEMISTRY
Journal acronym: ANAL CHEM
Volume: 69
Issue: 16
First page : 3385
Last page: 3390
Number of pages: 6
ISSN: 0003-2700
DOI: https://doi.org/10.1021/ac9611733
Abstract
Luminol shows strong chemiluminescence with an emission maximum at similar to 430 nm in the presence of sulfate radicals. Sulfate radicals were produced by the dissolution of UV-irradiated potassium peroxodisulfate powder in aqueous luminol solutions. The UV irradiation at 6.7 eV produces a solid solution of sulfate radical in potassium peroxodisulfate by rupturing -O-O- bonds, as in solution, but now the solid solution is stable in a time scale of years in dryness. In the present system, luminol chemiluminescence is produced via several parallel pathways having a common triggering step, one-electron oxidation of luminol monoanion by sulfate or hydroxyl radical. Present chemiluminescence allows sensitive luminol detection from picomolar to micromolar level with a linear response over 5 orders of magnitude, after which luminescence is too strong for single-photon counting. The high sensitivity of luminol detection allows us to propose extrinsic lyoluminescence of potassium peroxodisulfate as a new and simple method for detection step of bioaffinity assays using luminol or isoluminol derivatives as label compounds.
Luminol shows strong chemiluminescence with an emission maximum at similar to 430 nm in the presence of sulfate radicals. Sulfate radicals were produced by the dissolution of UV-irradiated potassium peroxodisulfate powder in aqueous luminol solutions. The UV irradiation at 6.7 eV produces a solid solution of sulfate radical in potassium peroxodisulfate by rupturing -O-O- bonds, as in solution, but now the solid solution is stable in a time scale of years in dryness. In the present system, luminol chemiluminescence is produced via several parallel pathways having a common triggering step, one-electron oxidation of luminol monoanion by sulfate or hydroxyl radical. Present chemiluminescence allows sensitive luminol detection from picomolar to micromolar level with a linear response over 5 orders of magnitude, after which luminescence is too strong for single-photon counting. The high sensitivity of luminol detection allows us to propose extrinsic lyoluminescence of potassium peroxodisulfate as a new and simple method for detection step of bioaffinity assays using luminol or isoluminol derivatives as label compounds.
UTU Research Portal