A1 Refereed original research article in a scientific journal
Inactivation of the androgen receptor in bone-forming cells leads to trabecular bone loss in adult female mice
Authors: Maatta JA, Buki KG, Ivaska KK, Nieminen-Pihala V, Elo TD, Kahkonen T, Poutanen M, Harkonen P, Vaananen K
Publisher: nature publishing group
Publishing place: England
Publication year: 2013
Journal: BoneKEy Reports
Journal name in source: BoneKEy reports
Journal acronym: Bonekey Rep.
Volume: 2
First page : 440
ISSN: 2047-6396
DOI: https://doi.org/10.1038/bonekey.2013.174
Abstract
Removal of the androgen receptor (AR) from bone-forming cells has been shown to reduce trabecular bone volume in male mice. In female mice, the role of AR in the regulation of bone homeostasis has been poorly understood. We generated a mouse strain in which the AR is completely inactivated only in mineralizing osteoblasts and osteocytes by breeding mice carrying osteocalcin promoter-regulated Cre-recombinase with mice possessing loxP recombination sites flanking exon 2 of the AR gene (AR(DeltaOB/DeltaOB) mice). In female AR(DeltaOB/DeltaOB) mice, the trabecular bone volume was reduced owing to a smaller number of trabeculae at 6 months of age compared with the control AR(fl/fl) animals. In male AR(DeltaOB/DeltaOB) mice, an increase in trabecular bone separation could already be detected at 3.5 months of age, and at 6 months, the trabecular bone volume was significantly reduced compared with that of male AR(fl/fl) mice. No AR-dependent changes were observed in the cortical bone of either sex. On the basis of micro-computed tomography and histomorphometry, we conclude that in male mice, the AR is involved in the regulation of osteoclast number by osteoblasts, whereas in female mice, the lack of the AR in the bone-forming cells leads to a decreased number of trabeculae upon aging.\nJID: 101586246; PMCR: 2014/11/06 00:00; 2013 [ecollection]; 2013/03/01 [received]; 2013/09/09 [accepted]; 2013/11/06 [epublish]; epublish
Removal of the androgen receptor (AR) from bone-forming cells has been shown to reduce trabecular bone volume in male mice. In female mice, the role of AR in the regulation of bone homeostasis has been poorly understood. We generated a mouse strain in which the AR is completely inactivated only in mineralizing osteoblasts and osteocytes by breeding mice carrying osteocalcin promoter-regulated Cre-recombinase with mice possessing loxP recombination sites flanking exon 2 of the AR gene (AR(DeltaOB/DeltaOB) mice). In female AR(DeltaOB/DeltaOB) mice, the trabecular bone volume was reduced owing to a smaller number of trabeculae at 6 months of age compared with the control AR(fl/fl) animals. In male AR(DeltaOB/DeltaOB) mice, an increase in trabecular bone separation could already be detected at 3.5 months of age, and at 6 months, the trabecular bone volume was significantly reduced compared with that of male AR(fl/fl) mice. No AR-dependent changes were observed in the cortical bone of either sex. On the basis of micro-computed tomography and histomorphometry, we conclude that in male mice, the AR is involved in the regulation of osteoclast number by osteoblasts, whereas in female mice, the lack of the AR in the bone-forming cells leads to a decreased number of trabeculae upon aging.\nJID: 101586246; PMCR: 2014/11/06 00:00; 2013 [ecollection]; 2013/03/01 [received]; 2013/09/09 [accepted]; 2013/11/06 [epublish]; epublish
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