A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Mutagenesis of the D-E loop of photosystem II reaction centre protein D1. Function and assembly of photosystem II
Tekijät: Mulo P, Tyystjarvi T, Tyystjarvi E, Govindjee, Maenpaa P, Aro EM
Kustantaja: SPRINGER
Julkaisuvuosi: 1997
Journal: Plant Molecular Biology
Tietokannassa oleva lehden nimi: PLANT MOLECULAR BIOLOGY
Lehden akronyymi: PLANT MOL BIOL
Vuosikerta: 33
Numero: 6
Aloitussivu: 1059
Lopetussivu: 1071
Sivujen määrä: 13
ISSN: 0167-4412
DOI: https://doi.org/10.1023/A:1005765305956
Tiivistelmä
The sequence connecting alpha-helices D and E of the D1 protein in photosystem II (PSII) is longer than that found in the corresponding loop of the L subunit in the rhodobacterial reaction centre. This sequence was mutated in order to determine its role in oxygenic photosynthesis. Site-specific mutants, including point mutations and deletions of different size, of the PEST-like region and the putative cleavage area in the D-E loop of the DI protein were constructed in Synechocystis sp. PCC 6803. The effects of mutations on the functional and structural properties of PSII and turnover of the DI protein were examined. Our results demonstrate that deletion of either the PEST-like sequence (Delta R225-F239) or the putative cleavage region (Delta G240-V249, Delta R225-V249) of the D1 protein resulted in severe perturbations on the function of the Q(B) electron acceptor of PSII. However, PSII centres of the mutant with deleted PEST region remained functional enough to support autotrophic growth whereas deletions of the putative cleavage region prevented autotrophic growth. Although enhanced degradation rates of the mutant D1 proteins under low-light growth conditions demonstrate that neither the PEST-like sequence nor the putative cleavage region are required for D1 proteolysis, it became clear that the extension in the D-E loop of the D1 protein is essential for proper PSII assembly and photoautotrophic growth. Moreover, modifications of the D-E loop resulted in transcriptional activation of the psbA gene, indicating that neither light intensity, as such, nor the activity of the electron transfer chain are the only determinants in regulation of psbA gene transcription.
The sequence connecting alpha-helices D and E of the D1 protein in photosystem II (PSII) is longer than that found in the corresponding loop of the L subunit in the rhodobacterial reaction centre. This sequence was mutated in order to determine its role in oxygenic photosynthesis. Site-specific mutants, including point mutations and deletions of different size, of the PEST-like region and the putative cleavage area in the D-E loop of the DI protein were constructed in Synechocystis sp. PCC 6803. The effects of mutations on the functional and structural properties of PSII and turnover of the DI protein were examined. Our results demonstrate that deletion of either the PEST-like sequence (Delta R225-F239) or the putative cleavage region (Delta G240-V249, Delta R225-V249) of the D1 protein resulted in severe perturbations on the function of the Q(B) electron acceptor of PSII. However, PSII centres of the mutant with deleted PEST region remained functional enough to support autotrophic growth whereas deletions of the putative cleavage region prevented autotrophic growth. Although enhanced degradation rates of the mutant D1 proteins under low-light growth conditions demonstrate that neither the PEST-like sequence nor the putative cleavage region are required for D1 proteolysis, it became clear that the extension in the D-E loop of the D1 protein is essential for proper PSII assembly and photoautotrophic growth. Moreover, modifications of the D-E loop resulted in transcriptional activation of the psbA gene, indicating that neither light intensity, as such, nor the activity of the electron transfer chain are the only determinants in regulation of psbA gene transcription.
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